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Use of fungicides in plant tissue culture

Abstract

We have examined a number of antifungal agents which might prove useful in plant tissue culture. We find that carbendazim, fenbendazole and imazalil can be used relatively safely and also have a broad spectrum of antifungal activity. Fungicides in clinical use do not prove to be as effective.

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Abbreviations

YEPD:

yeast potato dextrose, MS medium is Murashige and Skoog medium purchased from Flow Laboratories

2,4 D:

2,4 dichlorophenoxyacetic acid

BAP:

6-Benzylamino purine. Benomyl is methyl-(butylcarbamoyl)-2-benzimidazole carbamate

MBC:

is Carbendazim (methyl benzimidazole -2-yl-carbamate)

TBZ:

is Thiabendazole; 2-(Thiazol-4-y-l) benzimidazole

FBZ:

is Fenbendazole [5-(Phenylthio)-1 H-benzimidazole-2-yl] carbamic acid methyl ester

References

  • Bancroft, JB., F. Motoyoshi JW Watts and JRO Dawson (1975) In: Markham et al (eds) Modification of the information content of plant cells. North Holland Amsterdam pp 133

    Google Scholar 

  • Beggs, WH., GA Sarosi and MI Walker (1976) J Infect. Dis 133, 206–209.

    Google Scholar 

  • Chiu, PL., PJ Bottino and GW Patterson (1979). Lipids 15 50–54.

    Google Scholar 

  • Brown, DM., CL Groom, M. Cvitanik, M. Brown, JL Cooper and J Arditti (1982). Plant Cell Tissue and Organ Culture 1 165–180.

    Google Scholar 

  • Garrod, LP., HP Lambert, F O'Grady and PM Waterworth (1981). In: Antibiotic and Chemotherapy (5th ed). Churchill Livingstone, Edinburgh.

    Google Scholar 

  • Hammerschlag RS. and HD Sisler (1973) Pest. Biochem. Physiol. 3 42–54

    Google Scholar 

  • Kunze ME and P Todd (1983). In vitro 19 175–178.

    Google Scholar 

  • Laskey RA (1970). J Cell. Sci. 7 653–659 (1970).

    Google Scholar 

  • Lo Schiavo F., V. Nuti Ronchi and M. Terzi (1980). Theor. Appl. Genet. 58 43–47

    Google Scholar 

  • Medoff G. and GA Kobayashi (1980) In: D.C.E. Speller (ed) Antifungal Chemotherapy. John Wiley and Son, Chichester pp 3–33.

    Google Scholar 

  • Pollock K, DG Barfield and R. Shields (1983). Plant Cell Reports 2 36–39.

    Google Scholar 

  • Roy D. and PA Fantes (1982). Curr. Genet. 6 195–201.

    Google Scholar 

  • Schreiber LR and WK Hock (1975). J. Amer. Soc. Hort. Sci. 100 309–313.

    Google Scholar 

  • Seiler JP (1975). Mutation Res. 32 151–168.

    Google Scholar 

  • Van den Bossche H., G Willemsens, W Cools, F Conelissen, WF Lauwers and JM van Catsem (1980). Antimicrob. Agents and Chemother. 4 306–308.

    Google Scholar 

  • Voogd CE and JJ van der Stel (1983). Mut. Res. 120 91–95

    Google Scholar 

  • Watts JW and JM King (1973). Planta 113 271–277.

    Google Scholar 

  • Wood JJ (1982). Mol. Cell. Biol. 2 1064–1079.

    Google Scholar 

  • Worthing (1979). In: The Pesticide Manual, a world compendium (6th ed). British Crop Protection Council.

Download references

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Communicated by I. Potrykus

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Shields, R., Robinson, S.J. & Anslow, P.A. Use of fungicides in plant tissue culture. Plant Cell Reports 3, 33–36 (1984). https://doi.org/10.1007/BF00270226

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  • DOI: https://doi.org/10.1007/BF00270226

Keywords

  • Tissue Culture
  • Plant Tissue
  • Broad Spectrum
  • Antifungal Activity
  • Antifungal Agent