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In vitro morphogenesis from excised leaf explants of Digitalis obscura L.

Plant Cell Reports volume 3, pages 8–9 (1984)Cite this article

Abstract

The morphogenic capacity of Digitalis obscura leaf explants cultured in vitro has been studied, noting factors promoting the differentiation of roots, buds and shoots as well as those promoting callus proliferation. Complete plant regeneration was obtained only by first culturing the leaf explants in a medium with NAA and BA to induce formation of buds, and subsequently transferring them to a medium without growth regulators to achieve the further development of shoots.

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Abbreviations

BA:

benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indoleacetic acid

NAA:

naphthaleneacetic acid

References

  • Bhatt PN, Bhatt DP, Sussex IM (1979) Z Pflanzenphysiol 95: 355–362

    Google Scholar 

  • Dunwell JM (1981) J Exp Bot 32: 789–800

    Google Scholar 

  • Hagimori M, Matsumoto T, Kisaki T (1980) Plant Cell Physiol 21: 1391–1404

    Google Scholar 

  • Pérez-Bermúdez P, Cornejo MJ, Segura J (1983) Plant Sci Lett 30: 77–82

    Google Scholar 

  • Rucker W (1982) Z Pflanzenphysiol 107: 141–151

    Google Scholar 

  • Rucker W, Jentzsch K, Wichtl M (1976) Z Pflanzenphysiol 80: 323–335

    Google Scholar 

  • Rucker W, Jentzsch K, Wichtl M (1981) Z Pflanzenphysiol 102: 207–220

    Google Scholar 

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Author information

Affiliations

  1. Department of Plant Physiology, College of Pharmacy, University of Valencia, Avda. Blasco Ibañez 13, Valencia 10, Spain

    P. Pérez-Bermúdez, M. C. Brisa, M. J. Cornejo & J. Segura

Authors
  1. P. Pérez-Bermúdez
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  2. M. C. Brisa
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  3. M. J. Cornejo
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  4. J. Segura
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Additional information

Communicated by M. H. Zenk

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Pérez-Bermúdez, P., Brisa, M.C., Cornejo, M.J. et al. In vitro morphogenesis from excised leaf explants of Digitalis obscura L.. Plant Cell Reports 3, 8–9 (1984). https://doi.org/10.1007/BF00270218

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  • DOI: https://doi.org/10.1007/BF00270218

Keywords

  • Growth Regulator
  • Plant Regeneration
  • Leaf Explants
  • Callus Proliferation
  • Complete Plant