Abstract
Because most interspecific Medicago embryos abort before they can be excised and cultured, our objective was to grow young pods in vitro. Various media were used to grow three-day-old pods of annuals [diploids, M. blancheana Boiss., M. disciformis DC., tetraploid M. scutellata (L.) Mill.] and perennials (diploid M. falcata L., tetraploid M. sativa L.).
Few pods of perennial species grew to maturity on media containing modified Hoagland's plus 1% glucose or sucrose with or without 5% potato extract. Increasing sucrose to 6% increased the percentage of M. sativa pods that produced mature seeds. On DM (differentiation medium), the best medium, the percentage of pods producing viable seeds was: M. blancheana (82), M. disciformis (81), M. scutellata (48), M. sativa (63), M. falcata (15). DM plus 1 ppm indoleacetic or gibberellic acid did not enhance seed production.
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Abbreviations
- DM:
-
Differentiation medium
- DMI:
-
DM plus 1 ppm indoleacetic acid
- DMG:
-
DM plus 1 ppm gibberellic acid
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Communicated by J. Widholm
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Wang, J.W., Sorensen, E.L. & Liang, G.H. In vitro culture of pods from annual and perennial Medicago species. Plant Cell Reports 3, 146–148 (1984). https://doi.org/10.1007/BF00270209
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DOI: https://doi.org/10.1007/BF00270209