Abstract
The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of nitrate reductase activity followed Michaelian kinetics. The apparent Km values for NADH were 0.083 mM and 0.20 mM, respectively, for the dark- and the light-grown callus. In vivo nitrate reductase activity in green sugarcane leaves (field grown) was unaffected by NADH in the reaction system. Under the standard conditions of assay up to 60% of the NADH penetrated into the sugarcane callus within 2 min. No penetration of NADH into the sugarcane leaf discs was, however, recorded under identical conditions.
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Communicated by J. Widholm
NCL Communication No. 3454
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Khan, B.M., Dwivedi, U.N., Rawal, S.K. et al. In vivo nitrate reductase activity in dark- and light-grown sugarcane callus. Plant Cell Reports 3, 138–141 (1984). https://doi.org/10.1007/BF00270207
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DOI: https://doi.org/10.1007/BF00270207