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Amino acid pools and protein synthesis in germinating maize embryos

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Abstract

Amino acid pools were analyzed in maize (Zea mays L.) axes germinated for 0, 6 and 24 h. Proteins synthesized at early (0–6 h) and late (18–24 h) stages were characterized by gel electrophoresis and fluorography after either 14C-leucine or 14C-lysine pulses. An increase of amino acid incorporation after 18–24 h was observed, as well as changes in the protein patterns of the corresponding fluorographs. Analysis of the endogenous amino acid pools showed major changes in contents of proline, alanine, isoleucine, valine, leucine and lysine. A selective increase of lysine incorporation into proteins during the late stage was detected.

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Authors and Affiliations

  1. Plant Biochemistry Department, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, 04510, Mexico, DF

    Raul Aguilar & Estela Sanchez de Jiminez

Authors
  1. Raul Aguilar
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  2. Estela Sanchez de Jiminez
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Additional information

Communicated by O. L. Gamborg

Under the Academic Colaboration Program between the Universidad Nacional Autónoma de México and the Colegio de Postgraduados, Chapingo, México.

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Aguilar, R., Sanchez de Jiminez, E. Amino acid pools and protein synthesis in germinating maize embryos. Plant Cell Reports 3, 193–195 (1984). https://doi.org/10.1007/BF00270198

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  • DOI: https://doi.org/10.1007/BF00270198

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