Skip to main content
SpringerLink
Log in

Tropane alkaloid production in cultured cells of Duboisia leichhardtii

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Calluses were derived from explants of aerial parts of a Duboisia leichhardtii F. Muell. plant. After 3 months of culture we detected small amounts of hyoscyamine and scopolamine in the unorganized calluses, but the amounts decreased until they were undetectable with successive subculture. When shoots were produced from the unorganized calluses, the ability to convert hyoscyamine added into media to scopolamine appeared; when roots were initiated from the shoot-organizing calluses, hyoscyamine, scoporamine, and nicotine were synthesized. Cultured adventitious roots produced these alkaloids and converted exogenous hyoscyamine to scopolamine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

NAA:

1-naphthaleneacetic acid

BA:

benzyladenine

IBA:

indole-3-butyric acid

References

  • Cougoul N, Miginiac E, Cosson L (1979) Phytochemistry 18: 949–951.

    Google Scholar 

  • Eapen S, Rangan TS, Chadha MS, Heble MR (1978) Plant Science Letters 13: 83–89.

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158.

    Google Scholar 

  • Griffin WJ (1979) Naturwissenschaften 66: 58.

    Google Scholar 

  • Hashimoto T, Yamada Y (1983) Planta Med 47: 195–199.

    Google Scholar 

  • Hiraoka N, Tabata M (1973) Phytochemistry 12: 795–799.

    Google Scholar 

  • Kagei K, Sato T, Ogata Y, Toyoshima T, Matsuura N (1980) Yakugaku Zasshi 100: 574–585 (In Japanese).

    Google Scholar 

  • Kennedy GS (1971) Phytochemistry 10: 1335.

    Google Scholar 

  • Kitamura Y, Miura H, Sugii M (1980) Shoyakugaku Zasshi 34: 58–65.

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Physiol Plant 18: 100–127.

    Google Scholar 

  • Sippy KJ, Friedrich H (1975) Planta Med Suppl: 186–187.

  • Waller GR, Nowacki EK (1978) in: Alkaloid Biology and Metabolism in Plants. New York, Plenum Press.

    Google Scholar 

  • White PR (1963) in: The Cultivation of Animal and Plant Cells. New York, Ronald Press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Center for Cell and Tissue Culture, Kyoto University, Kyoto, Japan

    Yasuyuki Yamada & Tsuyoshi Endo

Authors
  1. Yasuyuki Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tsuyoshi Endo
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Constabel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamada, Y., Endo, T. Tropane alkaloid production in cultured cells of Duboisia leichhardtii . Plant Cell Reports 3, 186–188 (1984). https://doi.org/10.1007/BF00270196

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270196

Keywords

Search

Navigation