Abstract
Transformation of interphase microtubular cytoskeleton into initial mitotic spindle in early prophase and the reverse process in telophase were analysed with immunofluorescence techniques in endosperm cells of higher plants, Haemanthus Katherinae Bak. and Clivia nobilis Lindl. We have identified aster-like centers as intermediate basic microtubular structures directly involved in the reorganization of microtubules arrays both at the onset of mitosis and during telophase-interphase transition. These transitory microtubule converging centers determine spindle polarity in early prophase, they are replaced by diffuse poles during metaphase, and form again in anaphasetelophase. We conclude that rearrangement of microtubules during interphase-mitosis involves three superimposed processes: microtubule assembly/disassembly, active transport and reorientation of microtubules, changes in microtubule properties reflected in their lateral interaction during spindle development.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- MT(s):
-
microtubules
- IIF:
-
indirect immunofluorescence
References
Aubin JE (1981) In: Zimmermann A and Forer A (ed) Mitosis/Cytokinesis, Academic Press, pp 211–244
Bajer A, Mole-Bajer J (1972) Spindle dynamics and chromosome movements. Suppl. 3, Intern Rev Cytol pp 1–271
Bajer A, Mole-Bajer J (1982) In: Cold Spring Harbor Symposia on quantitative biology 46:263–283
Brinkley BR, Cartwright J (1971) J Cell Biol 50:416–431
Brinkley BR, Fuller GM, Highfield DP (1975) Proc Natl Acad Sc USA 72: 4981–4985
De Mey J, Moeremans M, Guens G, Nuydens R, De Brabander M (1981) Cell Biol Inter Rep 5: 889–899
De Mey J, Lambert AM, Bajer A, Moeremans M, De Brabander M (1982) Proc Natl Acad Sc USA 79: 1898–1902
Franke WW, Seib E, Osborn M, Weber K, Herth W, Falk H (1977) Cytobiologie 15: 24–48
Gunning B (1982) In: Lloyd (ed) The cytoskeleton in Plant Growth and Development, Academic Press, pp 229–292
Gunning B, Hardham AR, Hughes JE (1978) Planta (Berl) 143: 161–179
Johnson G, Gloria M, Nogueira Arauso GM, (1981) J Immunol Meth 43: 339–350
Lambert AM (1980) Chromosoma 76: 295–308
Lambert AM, Bajer A (1972) Chromosoma 39: 101–144
Lambert AM, Vantard M, Van Eldik L, De Mey J (1983) J Cell Biol (abstract) in press
Lloyd CW, Slabas AR, Powell AJ, Lowe SB (1980) Planta 147: 500–506
Lloyd CW, Slabas AR, Powell AJ, McDonald G, Badley R (1979) Nature 279: 239–241
Mole-Bajer J (1975) Cytobios 13: 117–140
Mole-Bajer J, Bajer A (1968) La Cellule 67: 257–265
Osborn M, Webster RE, Weber K (1978) J Cell Biol 21: R27-R34
Pickett-Heaps JD (1969) J. Ultrastr. Res. 27: 24–44
Roos P (1973) Chromosoma 40: 43–82
Schmit AC, Lambert AM (1981) Biol Cell 41: 24a
Weber K, Osborn M (1979) In: Roberts K, Hyams JS (ed) Microtubules, Academic Press pp 279–313
Wick S, Seagull RW, Osborn M, Weber K, Gunning BES (1981) J Cell Biol 89: 685–690
Wick S, Duniec J (1983) J Cell Biol 97: 235–243
Author information
Authors and Affiliations
Additional information
Communicated by P. Matile
Rights and permissions
About this article
Cite this article
Schmit, AC., Vantard, M., de Mey, J. et al. Aster-like microtubule centers establish spindle polarity during interphase — Mitosis transition in higher plant cells. Plant Cell Reports 2, 285–288 (1983). https://doi.org/10.1007/BF00270182
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270182