Summary
Macromolecular synthesis in an Escherichia coli mutant with a temperature-sensitive β′ subunit of RNA polymerase was analysed. At the non-permissive temperature ribosomal RNA synthesis is strongly reduced while messenger RNA synthesis is affected to only a slight extent. The overall protein synthesis is only slightly affected. We conclude that the β′ subunit is involved in promoter recognition and plays a role in transcriptional selectivity.
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Communicated by A. Böck
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Oostra, B.A., Kok, K., Van Vliet, A.J. et al. A mutation in the RNA polymerase β′ subunit causing depressed ribosomal RNA synthesis in Escherichia coli . Molec. Gen. Genet. 183, 54–58 (1981). https://doi.org/10.1007/BF00270138
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DOI: https://doi.org/10.1007/BF00270138