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Effect of recB21, uvrD3, lexA101 and recF143 mutations on ultraviolet radiation sensitivity and genetic recombination in ΔuvrB strains of Escherichia coli K-12

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Summary

The interaction of the recB21, uvrD3, lexA101, and recF143 mutations on UV radiation sensitization and genetic recombination was studied in isogenic strains containing all possible combinations of these mutations in a ΔuvrB genetic background. The relative UV radiation sensitivities of the multiply mutant strains in the ΔuvrB background were: recF recB lexA> recF recB uvrD lexA, recF recB uvrD>recA>recF uvrD lexA> recF recB, recF uvrD>recF lexA>recB uvrD lexA>recB uvrD> recB lexA, lexA uvrD>recB>lexA, uvrD>recF; three of these strains were more UV radiation sensitive than the uvrB recA strain. There was no correlation between the degree of radiation sensitivity and the degree of deficiency in genetic recombination. An analysis of the survival curves revealed that the recF mutation interacts synergistically with the recB, uvrD, and lexA mutations in UV radiation sensitization, while the recB, uvrD, and lexA mutations appear to interact additively with each other. We interpret these data to suggest that there are two major independent pathways for postreplication repair; one is dependent on the recF gene, and the other is dependent on the recB, uvrD, and lexA genes.

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References

  • Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, Edition 6. Microbiol Rev 44:1–56

    Google Scholar 

  • Barfknecht TR, Smith KC (1978) The involvement of DNA polymerase I in the postreplication repair of ultraviolet radiation-induced damage in Escherichia coli K-12. Mol Gen Genet 167:37–41

    Google Scholar 

  • Braun A, Grossman L (1974) An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from the uvrA and uvrB mutants. Proc Natl Acad Sci USA 71:1838–1842

    Google Scholar 

  • Brendel M, Haynes RH (1973) Interactions among genes controlling sensitivity to radiation and alkylation in yeast. Mol Gen Genet 125:197–216

    Google Scholar 

  • Clark AJ (1967) The beginning of a genetic analysis of recombination proficiency. J Cell Physiol (Suppl.) 70:165–180

    Google Scholar 

  • Clark AJ (1980) A view of the recBC and recF pathways of E. coli recombination. In: Alberts B, Fox CF (eds) Mechanistic studies of DNA replication and genetic recombination. Academic Press, New York, pp 891–899

    Google Scholar 

  • Ganesan AK (1974) Persistence of pyrimidine dimers during postreplication repair in ultraviolet light-irradiated Escherichia coli. J Mol Biol 87:103–119

    Google Scholar 

  • Ganesan AK, Seawell PC (1975) The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12. Mol Gen Genet 141:189–205

    Google Scholar 

  • Ganesan AK, Smith KC (1968) Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec − mutations on liquid holding recovery. J Bacteriol 96:365–373

    Google Scholar 

  • Ganesan AK, Smith KC (1970) Dark recovery processes in Escherichia coli irradiated with ultraviolet light. III. Effect of rec mutations on recovery of excision-deficient mutants of Escherichia coli K-12. J Bacteriol 102:404–410

    Google Scholar 

  • Horii ZI, Clark AJ (1973) Genetic analysis of the recF pathway of genetic recombination in Escherichia coli K-12. Isolation and characterization of mutants. J Mol Biol 80:327–344

    Google Scholar 

  • Howard-Flanders P (1968) DNA repair. Ann Rev Biochem 37:175–200

    Google Scholar 

  • Howard-Flanders P, Boyce RP (1966) DNA repair and genetic recombination: Studies on mutants of Escherichia coli defective in these processes. Radiat Res Suppl. 6:156–184

    Google Scholar 

  • Howard-Flanders P, Boyce RP, Theriot L (1966) Three loci in Escherichia coli that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53:1119–1136

    Google Scholar 

  • Johnson BF (1977) Genetic mapping of the lexC-113 mutation. Mol Gen Genet 157:91–97

    Google Scholar 

  • Kato T (1977) Effects of chloramphenicol and caffeine on postreplication repair in uvrA − umuC −and uvrA − recF − strains of Escherichia coli K-12. Mol Gen Genet 156:115–120

    Google Scholar 

  • Kato T, Shinoura Y (1977) Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Mol Gen Gent 156:121–131

    Google Scholar 

  • Kato T, Rothman RH, Clark AJ (1977) Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by UV irradiation. Genetics 87:1–18

    Google Scholar 

  • Marinus MG, Morris NR (1975) Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. Mutat Res 28:15–26

    Google Scholar 

  • Mattern IE, Zwenk H, Rorsch A (1966) The genetic constitution of the radiation sensitive mutant Escherichia coli Bs-1. Mutat Res 3:374–380

    Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Ogawa H, Shimada K, Tomizawa J (1968) Studies on radiation sensitive mutants of Escherichia coli. I. Mutants defective in the repair synthesis. Mol Gen Genet 101:227–244

    Google Scholar 

  • Radman M (1974) Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis. In: Prakash L, Sherman F, Miller MW, Lawrence CW, Taber HW (eds) Molecular and environmental aspects of mutagenesis. CC Thomas, Springfield, Illinois, pp 128–142

    Google Scholar 

  • Rosamond J, Telander KM, Linn S (1979) Modulation of the action of the recBC enzyme of Escherichia coli by Ca2+. J Biol Chem 254:8646–8652

    Google Scholar 

  • Rothman RH, Clark AJ (1977a) Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12. Mol Gen Genet 155:267–277

    Google Scholar 

  • Rothman RH, Clark AJ (1977b) The dependence of postreplication repair on uvrB in a recF mutant of Escherichia coli K-12. Mol Gen Genet 155:279–286

    Google Scholar 

  • Rothman RH, Kato T, Clark AJ (1975) The beginning of an investigation of the role of recF in the pathways of metabolism of ultraviolet-irradiated DNA in Escherichia coli. In: Hanawalt PC, Setlow RB (eds) Molecular mechanisms for repair of DNA. Plenum Publishing Corporation, New York, pp 283–291

    Google Scholar 

  • Rupp WD, Wilde III CE, Reno DL, Howard-Flanders P (1971) Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol 61:25–44

    Google Scholar 

  • Sedgwick SG (1976) Mistepair of overlapping daughter strand gaps as a possible mechanism for UV induced mutagenesis in uvr strains of Escherichia coli: A general model for induced mutagenesis by misrepair (SOS repair) of closely spaced DNA lesions. Mutat Res 41:185–200

    Google Scholar 

  • Smith KC, Meun DHC (1970) Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on postreplication repair of damage due to ultraviolet radiation. J Mol Biol 51:459–472

    Google Scholar 

  • Stacey KA, Simson E (1965) Improved method for the isolation of thymine-requiring mutants of Escherichia coli. J Bacteriol 90:554–555

    Google Scholar 

  • Van der Schueren E, Youngs DA, Smith KC (1974) Sensitization of ultraviolet-irradiated Escherichia coli K-12 by different agars: Inhibition of a rec and exr gene-dependent branch of the uvr gene-dependent excision-repair process. Photochem Photobiol 20:9–13

    Google Scholar 

  • Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907

    Google Scholar 

  • Youngs DA, Smith KC (1976) Genetic control of multiple pathways of post-replicational repair in uvrB strains of Escherichia coli K-12. J Bacteriol 125:102–110

    Google Scholar 

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Authors and Affiliations

  1. Department of Radiology, Stanford University of School of Medicine, 94305, Stanford, California, USA

    Tzu-chien V. Wang & Kendric C. Smith

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  1. Tzu-chien V. Wang
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  2. Kendric C. Smith
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Communicated by B.A. Bridges

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Wang, Tc.V., Smith, K.C. Effect of recB21, uvrD3, lexA101 and recF143 mutations on ultraviolet radiation sensitivity and genetic recombination in ΔuvrB strains of Escherichia coli K-12. Molec. Gen. Genet. 183, 37–44 (1981). https://doi.org/10.1007/BF00270135

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  • DOI: https://doi.org/10.1007/BF00270135

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