Abstract
Immature embryos and immature leaf tissues were used to establish embryogenic cultures of Zea diploperennis. Callus was induced on media containing MS salts and vitamins, sucrose (2% for leaves, 6% for embryos), 5% coconut milk and 1–6 mg/l 2, 4-D. Embryogenic callus was maintained by subculturing on media containing MS salts and vitamins, 2% sucrose, 500 mg/l casein hydrolysate and 1 mg/l 2,4-D. Regeneration occurred when the 2,4-D level was reduced to 0.25 mg/l. Kinetin added at 0.25 mg/l further stimulated regeneration. Root tip squashes on 10 plants regenerated after 2 years in culture indicated a normal 2n=20 chromosome number.
Similar content being viewed by others
References
Botti C, Vasil IK (1983) Z. Pflanzenphysiol. 111: 319–325
Boyes CJ, Vasil IK (1984) Plant Sci. Lett. 35: 153–157
Brettell RIS, Wernicke, W, Thomas E (1980) Protoplasma 104: 141–145
Chandler SF, Vasil IK (1984) J. Plant Physiol. 117: 147–156
Dale PJ, Dalton SJ (1983) Z. Pflanzenphysiol. 111: 39–45
Duncan DR, Williams ME, Zehr BE, Widholm JM (1985) Planta 165: 322–332
Gamborg OL, Constabel F, Miller RA (1970) Planta 95: 355–358
Gray DJ, Conger BV (1985) Plant Cell Tiss. Org. Cult. 4: 123–133
Green CE (1982) In: Fujiwara A (ed) Proc. 5th Int. Congr. Plant Tissue Culture, Maruzen Co., Ltd., Tokyo, pp 107–108
Hanna WW, Lu C, Vasil IK (1984) Theor. Appl. Genet. 67: 155–159
Hanning GE, Conger BV (1982) Theor. Appl. Genet. 63: 155–159
Heyser JW, Nabors MW (1982) Crop Sci. 22: 1070–1074
Iltis HH (1983) Science 222: 886–894
Iltis HH, Doebley JF, Guzman MR, Pazy B (1979) Science 203: 186–188
Lu C, Vasil IK, Ozias-Akins P (1982) Theor. Appl. Genet. 62: 109–112
Lu C, Vasil V, Vasil IK (1983) Theor. Appl. Genet. 66: 285–289
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497
Nabors MW, Heyser JW, Dykes TA, DeMott KJ (1983) Planta 157: 385–391
Ozias-Akins P, Vasil IK (1982) Protoplasma 110: 95–105
Ozias-Akins P, Vasil IK (1983a) Protoplasma 115: 104–113
Ozias-Akins P, Vasil IK (1983b) Protoplasma 117: 40–44
Prioli LM, Silva WJ, Sondahl MR (1984) J. Plant Physiol. 117: 185–190
Sun CS, Chu CC (1986) Plant Cell Tiss. Org. Cult. 5: 175–178
Swedlund B, Vasil IK (1985) Theor. Appl. Genet. 69: 575–581
Tomes DT, Smith OS (1985) Theor. Appl. Genet. 70: 505–509
Vasil IK (1983) In: Lurquin, P, Kleinhofs, A (eds) Genetic Engineering in Eukaryotes, Plenum, New York pp 233–252
Vasil IK (1985) In: Henke, RR, Hughes, KW, Constantin, MP, Hollaender, A (eds) Tissue Culture in Forestry and Agriculture, Plenum, New York pp 31–47
Vasil V, Vasil IK (1981) Amer. J. Bot. 68: 864–872
Vasil V, Vasil IK (1982) Bot. Gaz. 143: 454–465
Author information
Authors and Affiliations
Additional information
Communicated by I.K. Vasil
Rights and permissions
About this article
Cite this article
Swedlund, B., Locy, R.D. Somatic embryogenesis and plant regeneration in two-year old cultures of Zea diploperennis . Plant Cell Reports 7, 144–147 (1988). https://doi.org/10.1007/BF00270126
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00270126