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Plant Cell Reports

, Volume 7, Issue 2, pp 130–133 | Cite as

Tissue culture of Chrysosplenium americanum and its potential for flavonoid production

  • L. Brisson
  • R. K. Ibrahim
  • M. Rideau
Article

Abstract

Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface sterilization procedure, (b) production of new shoots from initial explants, and (c) selective elimination of organogenic structures during several subcultures. HPLC analysis of the cultured tissues established the presence of a number of flavonoids characteristic of the intact plant, though in lower and variable concentrations. However, shoot and callus cultures exhibited flavonoid profiles similar to those of the intact leaves and roots, respectively.

Keywords

Special Surface Flavonoid HPLC Analysis Glucoside Flavonol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

benzylamino purine

2,4-D

2,4-dichlorophenoxyacetic acid

IAA

3-indoleacetic acid

NAA

1-naphtaleneacetic acid

HPLC

high pressure liquid chromatography

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References

  1. Ammirato P.V. (1983) In: Handbook of Plant Cell Culture, vol. 3 (Ammirato P.V., D.A. Evans, W.R. Sharp and Y. Yamada, eds.), pp. 327–354, Macmillan Publishing Company, New York.Google Scholar
  2. Ayabe S.I., M. Kobayashi, M. Hikichi, K. Matsumoto and T. Furuya (1980) Phytochemistry 19: 2179–2183.Google Scholar
  3. Bohm H. (1980) Int. Rev. Cytol. (Suppl.) 11B: 183–208.Google Scholar
  4. Brunet G. and R.K. Ibrahim (1973) Z. Pflanzenphysiol. 69: 152–162.Google Scholar
  5. Collins F.W., V. De Luca, R.K. Ibrahim, B. Voirin and M. Jay (1981) Z. Naturforsch. 36c: 730–736.Google Scholar
  6. Gamborg O.L., R.A. Miller and K. Ojima (1968) Exp. Cell Res. 50: 151–158.Google Scholar
  7. Hahlbrock K. (1977) In: Plant Tissue Culture and Its Biotechnological Application, (Barz, W., E. Reinhard and M.H. Zenk, eds.), pp. 95–122. Springer-Verlag, NY.Google Scholar
  8. Heller R. (1953) Ann. Sci. Nat. Bot. 14: 1–223.Google Scholar
  9. Ibrahim R.K. (1987) In: Cell Culture and Somatic Cell Genetics of Plants, vol. 4 (F. Constabel and I. Vasil, eds.) pp. 85–97, Academic Press, NY.Google Scholar
  10. Ibrahim R.K., H. Khouri, L. Brisson, L. Latchinian, D. Barron and L. Varin (1987) Bull. Liaison Groupe Polyphenols 13: 3–14.Google Scholar
  11. Jalal M.A.F. and H.A. Collin (1977) Phytochemistry 16: 1377–1380.Google Scholar
  12. Martin J.P., S. Cas and H. Rabechault (1972) Oleagineux 27: 303–305.Google Scholar
  13. Muhitch M.J. and J.S. Fletcher (1985) Plant Physiol. 78: 25–28.Google Scholar
  14. Murashige T. and F. Skoog (1962) Physiol. Plant. 15: 473–497.Google Scholar
  15. Newmann D., G. Krauss and D. Groger (1983) Planta medica 48: 20–23.Google Scholar
  16. Nishi T., Y. Yamada and E. Takahashi (1968) Nature 219: 502–509.Google Scholar
  17. Samejima M. and T. Yoshimoto (1983) In: International Symposium of Wood and Pulping Chemistrv. vol. 1 pp. 111–114, Tsukaba, Japan.Google Scholar
  18. Skirvin S. (1983) In: Handbook of Plant Cell Culture Vol.3 (Ammirato P.V., D.A. Evans, W.R. Sharp and Y. Yamada, eds.) pp. 402–452, Macmillan Publishing Company, NY.Google Scholar
  19. Treutter D., R. Galensa, W. Feucht and P.P.S. Schmid (1985) Physiol. Plant. 65: 95–101.Google Scholar
  20. Yamamoto H., N. Chatani, A. Kitayama and T.Y. Tomimori (1986) Plant Cell Tissue Organ Culture 5: 219–222.Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • L. Brisson
    • 1
  • R. K. Ibrahim
    • 1
  • M. Rideau
    • 2
  1. 1.Plant Biochemistry Laboratory, Department of BiologyConcordia UniversityMontrealCanada
  2. 2.Laboratoire de Biotechnologie vegetale, Faculté de PharmacieToursFrance

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