Plant Cell Reports

, Volume 7, Issue 2, pp 130–133 | Cite as

Tissue culture of Chrysosplenium americanum and its potential for flavonoid production

  • L. Brisson
  • R. K. Ibrahim
  • M. Rideau


Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface sterilization procedure, (b) production of new shoots from initial explants, and (c) selective elimination of organogenic structures during several subcultures. HPLC analysis of the cultured tissues established the presence of a number of flavonoids characteristic of the intact plant, though in lower and variable concentrations. However, shoot and callus cultures exhibited flavonoid profiles similar to those of the intact leaves and roots, respectively.


Special Surface Flavonoid HPLC Analysis Glucoside Flavonol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



benzylamino purine


2,4-dichlorophenoxyacetic acid


3-indoleacetic acid


1-naphtaleneacetic acid


high pressure liquid chromatography


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • L. Brisson
    • 1
  • R. K. Ibrahim
    • 1
  • M. Rideau
    • 2
  1. 1.Plant Biochemistry Laboratory, Department of BiologyConcordia UniversityMontrealCanada
  2. 2.Laboratoire de Biotechnologie vegetale, Faculté de PharmacieToursFrance

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