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Tissue culture of Chrysosplenium americanum and its potential for flavonoid production

Plant Cell Reports volume 7, pages 130–133 (1988)Cite this article

Abstract

Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface sterilization procedure, (b) production of new shoots from initial explants, and (c) selective elimination of organogenic structures during several subcultures. HPLC analysis of the cultured tissues established the presence of a number of flavonoids characteristic of the intact plant, though in lower and variable concentrations. However, shoot and callus cultures exhibited flavonoid profiles similar to those of the intact leaves and roots, respectively.

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Abbreviations

BAP:

benzylamino purine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

3-indoleacetic acid

NAA:

1-naphtaleneacetic acid

HPLC:

high pressure liquid chromatography

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Author information

Affiliations

  1. Plant Biochemistry Laboratory, Department of Biology, Concordia University, H3G 1M8, Montreal, Quebec, Canada

    L. Brisson & R. K. Ibrahim

  2. Laboratoire de Biotechnologie vegetale, Faculté de Pharmacie, F-3700, Tours, France

    M. Rideau

Authors
  1. L. Brisson
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  2. R. K. Ibrahim
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  3. M. Rideau
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Additional information

Communicated by F. Constabel

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Brisson, L., Ibrahim, R.K. & Rideau, M. Tissue culture of Chrysosplenium americanum and its potential for flavonoid production. Plant Cell Reports 7, 130–133 (1988). https://doi.org/10.1007/BF00270122

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  • DOI: https://doi.org/10.1007/BF00270122

Keywords

  • Special Surface
  • Flavonoid
  • HPLC Analysis
  • Glucoside
  • Flavonol