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Cell division and differentiation in protoplasts from cell cultures of Glycine species and leaf tissue of soybean

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Abstract

Protoplasts were isolated from cell cultures of G. soja and G. tabacina, respectively. The isolation procedure employed Percoll for the separation and concentration of protoplasts. The cultured protoplasts formed cells which developed into embryo-like structures. Protoplasts also were isolated from leaf tissue of soybean cv. Williams 82. Upon culture, the protoplasts regenerated cell walls and divided to form cell cultures.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid BA|Benzyladenine

BA:

Benzyladenine

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Authors and Affiliations

  1. International Plant Research Institute, 94070, San Carlos, CA, USA

    O. L. Gamborg, B. P. Davis & R. W. Stahlhut

  2. Health Science Center, University of Texas, Dallas, TX, USA

    B. P. Davis

  3. Department of Agronomy, University of Illinois, Urbana, IL, USA

    R. W. Stahlhut

  4. Genentech Inc., 460 Point San Bruno Blvd., 94080, South San Francisco, CA, USA

    O. L. Gamborg

Authors
  1. O. L. Gamborg
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  2. B. P. Davis
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  3. R. W. Stahlhut
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Gamborg, O.L., Davis, B.P. & Stahlhut, R.W. Cell division and differentiation in protoplasts from cell cultures of Glycine species and leaf tissue of soybean. Plant Cell Reports 2, 213–215 (1983). https://doi.org/10.1007/BF00270107

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  • DOI: https://doi.org/10.1007/BF00270107

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