Abstract
Induction of crown galls on 4–6 week old soybean (Glycine max (L.) Merr.) plants cultured in growth chambers was obtained with Agrobacterium tumefaciens strains C58, T37 and ACH5. The crown galls were isolated and cultured in vitro as sterile callus and liquid suspension cultures. Transformation was tested by opine tests and by molecular hybridization of restricted cell DNA with T-DNA fragments. Protoplasts were isolated from suspension cultures. Transformed protoplasts regenerate cell walls, divide and form calli without an exogenous supply of hormones.
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References
Aerts M, Jacobs M, Hernalsteens JP, Van Montagu M, Schell J (1979) Plant Sci Lett 17: 43–50.
Anderson AR, Moore L (1979) Phytopathology 69: 320–323.
Beversdorf WD, Bingham ET (1977) Crop Sci 17: 307–311.
Birnboim HC, Doly J (1979) Nucl Acids Res 7:1513–1523.
Braun AC (1956) Cancer Res 16: 53–56.
Chilton M-D, Drummond HJ, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Cell 11: 263–271.
Chilton M-D, Saiki RK, Yadav N, Gordon MP, Quetier F (1980) Proc Nat Acad Sci USA 77: 4060–4064.
De Cleene M, Ley JDe (1976) Bot Rev 42: 389–466.
Davey MR, Cocking EC, Freeman J, Pearce N, Tudor I (1980) Plant Sci Lett 18: 307–313.
Denhardt DT (1966) Biochem Biophys Res Commun 23: 641–652.
Depicker A, Wilde M De, Vos G De, Vos R De, Montagu M Van, Schell J (1980) Plasmid 3: 193–211.
Drummond MH, Gordon MP, Nester EW, Chilton M-D (1977) Nature 269: 535–536.
Drummond MH (1979) Nature 281: 343–347.
Gurley WB, Kemp JD, Albert MJ, Sutton DW, Callis J (1979) Proc Natl Acad Sci USA 76: 2828–2832.
Hirsch PR, Montagu M Van, Johnston AWB, Brewin JN, Schell J (1980) J Gen Microbiol 120: 403–412.
Hudges J, McColly ME (1975) Stain Techn 50: 319–329.
Kao KN, Michayluk MR (1975) Planta 126: 105–110.
Katekar GF, Geissler AE (1980) Plant Physiol 66: 1190–1195.
Klein AS, Montezinos D, Delmer DP (1981) Planta 152: 105–114.
Larkin PJ, Scowcroft WR, Geissler AE, Katekar GF (1982) Aust J Plant Physiol 9: 297–307.
Leemans J, Deblaere R, Willmitzer L, Greve H De, Hernalsteens JP, Montagu M Van, Schell J (1982) EMBO J 1: 147–152.
Lemmers M, Beuckeleer M De, Holsters M, Zambryski P, Depicker A, Hernalsteens JP, Montagu M Van, Schell J (1980) J Mol Biol 144: 353–376.
Matthysse AG, Gurlitz RHG (1982) Physiol Plant Pathol 21: 381–387.
Miller CO (1968) In: Wightman F & Settersfield G (eds) Biochemistry and Physiology of Plant Growth Substances, The Runge Press Ltd., Ottowa, pp. 33–45.
Petit A, Delhaye S, Tempe J, Morel G (1970) Physiol Veg 8: 205–213.
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113: 237–251.
Schell J, Montagu M Van, Beuckeleer M De, Block M De, Depicker A, Wilde M De, Engler G, Genetello C, Hernalsteens JP, Holsters M, Seurinck J, Silva B, Vliet F Van, Villarroel R (1979) Proc R Soc London Ser B 204: 251–266.
Scowcroft WR, Davey MR, Power JB (1973) Plant Sci Lett 1: 451–456.
Southern EM (1975) J Mol Biol 98: 503–517.
Van Larebeke N, Engler G, Holsters M, Elsacker S Van den, Zaenen I, Schilperoort RA, Schell J (1974) Nature 252: 169–170.
Watson B, Currier TC, Gordon MP, Chilton M-D, Nester EW (1975) J Bacteriol 123: 255–264.
Willmitzer L, Beuckeleer M De, Lemmers M, Montagu M Van, Schell J (1980) Nature 287: 359–361.
Wullems GJ, Molendijk L, Schilperoort RA (1980) Theor Appl Gen 56: 203–208.
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Communicated by D. von Wettstein
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Pedersen, H.C., Christiansen, J. & Wyndaele, R. Induction and in vitro culture of soybean crown gall tumors. Plant Cell Reports 2, 201–204 (1983). https://doi.org/10.1007/BF00270104
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DOI: https://doi.org/10.1007/BF00270104