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Haploid plants from in vitro anther culture of Annona squamosa Linn

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Abstract

Haploid plants were induced from anther callus of Annona squamosa Linn. (Custard apple) on a Nitsch basal medium supplemented with 6-benzyl-aminopurine and naphthalene acetic acid. When naphthalene acetic acid was replaced by indole-3-acetic acid only multiple shoots were obtained. Pretreatment (chilling, centrifugation, reduced atmospheric pressure etc.) of the flowers was not effective but dissection of the flowers in a suspension of activated charcoal and sucrose was found essential. The anthers required an initial dark period and a high sucrose medium followed by light and lowered sucrose levels. Root tip squashes of the regenerated plantlets revealed the haploid (n = 7) nature of the plantlets.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indo le-3-acetic acid

NAA:

α-naphthalene acetic acid

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Authors and Affiliations

  1. Biochemistry Division, National Chemical Laboratory, Pune 411 008, India

    S. Nair, P. K. Gupta & A. F. Mascarenhas

Authors
  1. S. Nair
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  2. P. K. Gupta
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  3. A. F. Mascarenhas
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Additional information

Communicated by F. Constabel

Communication No. 3187

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Nair, S., Gupta, P.K. & Mascarenhas, A.F. Haploid plants from in vitro anther culture of Annona squamosa Linn. Plant Cell Reports 2, 198–200 (1983). https://doi.org/10.1007/BF00270103

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  • DOI: https://doi.org/10.1007/BF00270103

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