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Callus formation from protoplasts of cultured Lithospermum erythrorhizon cells

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Abstract

Protoplasts isolated from cell cultures of Lithospermum erythrorhizon divided repeatedly and formed callus colonies. Factors that affect protoplast division are the use of glucose as osmoticum, a new plating method with twin layers of agar-liquid medium, and the culture of protoplasts under the osmolarity lower than that in the isolation solution. When the sucrose in the protoplast-culture medium was replaced with glucose, and coconut milk was added to the medium, the frequency of colony formation markedly increased. The culture period required for colony formation also was shortened.

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Author notes

  1. Y. Maeda

    Present address: Research Center, Mitsui Petrochemical Ind., Ltd., Waki-cho, Kuga-gun, 740, Yamaguchi, Japan

Authors and Affiliations

  1. Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan

    Y. Maeda & Y. Yamada

  2. Research Center, Mitsui Petrochemical Ind., Ltd., Waki-cho, Kuga-gun, 740, Yamaguchi, Japan

    Y. Fujita

Authors
  1. Y. Maeda
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  2. Y. Fujita
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  3. Y. Yamada
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Communicated by J. Widholm

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Maeda, Y., Fujita, Y. & Yamada, Y. Callus formation from protoplasts of cultured Lithospermum erythrorhizon cells. Plant Cell Reports 2, 179–182 (1983). https://doi.org/10.1007/BF00270097

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  • DOI: https://doi.org/10.1007/BF00270097

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