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A new procedure for increasing efficiency of protoplast plating and clone selection

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Abstract

Sea PrepTM agarose (FMC corp.) was used as a gelling agent for media in the culture of potato and tomato protoplasts. The concentration and conditions appropriate for gelling and liquifaction were determined. The new method provided increased calli transfer efficiency of up to 50-fold. Shoot formation from the protoplast-derived cells occurred normally and without reduction in frequency.

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Abbreviations

MS:

Murashige and Skoog (1962) medium

WT:

Wild type

CG:

Crown gall cultures of L. peruvianum

BA:

Benzyladenine

NAA:

Naphthaleneacetic acid

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Author information

Authors and Affiliations

  1. International Plant Research Institute, 94070, San Carlos, CA, USA

    T. L. Adams & J. A. Townsend

  2. Advanced Genetic Sciences, Berkeley, CA, USA

    J. A. Townsend

Authors
  1. T. L. Adams
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  2. J. A. Townsend
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Additional information

Communicated by O. L. Gamborg

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Adams, T.L., Townsend, J.A. A new procedure for increasing efficiency of protoplast plating and clone selection. Plant Cell Reports 2, 165–168 (1983). https://doi.org/10.1007/BF00270093

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  • DOI: https://doi.org/10.1007/BF00270093

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