Abstract
Chenopodium rubrum photoautotrophic cell suspensions were grown in plastic tissue culture dishes under photoautotrophic conditions. Growth was monitored by measuring cell number, packed cell volume, chlorophyll content and oxygen production. Such microtiter dishes are suitable systems for the serial assay of growth inhibition and various physiological effects (i.e. chlorophyll fluorescence, cell viability, oxygen production) of photoautotrophic cells as caused by herbicides and fungal phytotoxins. The applicability of the test system is discussed.
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Abbreviations
- pcv:
-
packed cell volume
- fr.w.:
-
fresh weight
- rpm:
-
revol. per minute
- DMSO:
-
dimethyl sulfoxide
- PMS:
-
phenazine methosulfate
- NBT:
-
nitro-blue tetrazolium chloride
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Communicated by E. W. Weiler
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Thiemann, J., Nieswandt, A. & Barz, W. A microtest system for the serial assay of phytotoxic compounds using photoautotrophic cell suspension cultures of Chenopodium rubrum . Plant Cell Reports 8, 399–402 (1989). https://doi.org/10.1007/BF00270078
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DOI: https://doi.org/10.1007/BF00270078