Summary
A study has been made of the regulation of the synthesis of Pl double-stranded (ds) RNA, the genome of the yeast virus-like particle. When yeast protein synthesis is prevented by starvation for a required amino acid or by addition of cycloheximide, the rate of Pl dsRNA synthesis is reduced markedly. During nitrogen starvation the synthesis of Pl dsRNA persists but is accompanied by the degradation of pre-existing molecules. This degradation appears to require the induction of new enzymes and it is likely that the breakdown products are used to enable the cell to complete its division cycle. However, all of the copies of the VLP genome are not degraded in this process, some are conserved and can replenish the amount of Pl dsRNA on return to growth conditions. The controls which must operate on Pl dsRNA synthesis are discussed and compared with those exerted on nuclear RNA synthesis in yeast.
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References
Baltimore, D.: Purification and properties of poliovirus doublestranded ribonucleic acid. J. Mol. Biol. 18, 421–428 (1966)
Bevan, E.A., Herring, A.J., Mitchell, D.J.: Two species of dsRNA in yeast; their preliminary characterisation and relationship to the killer character. Nature 245, 81–86 (1973)
Buck, K.W., Lhoas, P., Street, B.K.: Virus-like particles in yeast. Biochem. Soc. Trans. 1, 1141–1142 (1973)
Buck, K.W., Ratti, G.: A model for the replication of doublestranded ribonucleic acid mycoviruses. Biochem. Soc. Trans. 3, 542–544 (1975)
Fink, G.R.: The biochemical genetics of yeast. Methods Enzymol. 17 (A), 59–78 (1970)
Garvik, B., Haber, J.E.: New cytoplasmic genetic element that controls 20S RNA synthesis during sporulation in yeast. J. Bacteriol. 134, 261–269 (1978)
Herring, A.J., Bevan, E.A.: Virus-like particles associated with double-stranded RNA species found in killer and sensitive strains of yeast. J. Gen. Virol. 22, 387–439 (1974)
Herring, A.J., Bevan, E.A.: Yeast virus-like particles possess a capsid-associated single-stranded RNA polymerase. Nature 268, 464–466 (1977)
Holm, C.A., Oliver, S.G., Newman, A.M., Holland, L.E., McLaughlin, C.S., Wagner, E.K., Warner, R.C.: The molecular weight of yeast P1 double-stranded RNA. J. Biol. Chem. 253, 8332–8336 (1978)
Hopper, J.E., Bostian, K.A., Rowe, L.B., Tipper, D.J.: Translation of the L-species dsRNA genome of killer-associated virus-like particles of Saccharomyces cerevisiae. J. Biol. Chem. 252, 9010–9017 (1977)
Johnston, G.C., Pringle, J.R., Hartwell, L.H.: Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp. Cell Res. 105, 79–98 (1977a)
Johnston, G.C., Singer, R.A., McFarlane, E.S.: Growth and cell division during nitrogen starvation of the yeast Saccharomyces cerevisiae. J. Bacteriol. 132, 723–730 (1977b)
Johnston, L.H., Game, J.C.: Mutants of yeast with depressed DNA synthesis. Mol. Gen. Genet. 161, 205–214 (1978)
Livingston, D.M.: Inheritance of 2 μm DNA plasmid from Saccharomyces. Genetics 86, 73–84 (1977)
Ludwig, J.R. II, Oliver, S.G., McLaughlin, C.S.: The regulation of RNA synthesis in yeast II: Amino acids shift-up experiments. Mol. Gen. Genet. 158, 117–122 (1977)
Oliver, S.G., McCready, S.J., Holm, C., Sutherland, P.A., McLaughlin, C.S., Cox, B.S.: Biochemical and physiological studies of the yeast virus-like particle. J. Bacteriol. 130, 1303–1309 (1977)
Oliver, S.G., McLaughlin, C.S.: The regulation of RNA synthesis in yeast I: Starvation experiments. Mol. Gen. Genet. 154, 145–153 (1977)
Shulman, R.W., Sripati, C.E., Warner, J.R.: Non-coordinated transcription in the absence of protein synthesis in yeast. J. Biol. Chem. 252, 1344–1349 (1977)
Waldron, C.: Synthesis of ribosomal and transfer ribonucleic acids in yeast during nutritional shift-up. J. Gen. Microbiol. 98, 215–221 (1977)
Watson, T.G.: Amino acid pool composition of Saccharomyces cerevisiae as a function of growth rate and amino acid nitrogen source. J. Gen. Microbiol. 96, 263–268 (1976)
Weber, H., Lindner, R.: Virusartige partikeln in hefesprotoplasten I: Elektronmikroskopischer nachweis. Z. Allg. Mikrobiol. 15, 631–638 (1975)
Wehr, C.T., Parks, L.W.: Macromolecular synthesis in Saccharomyces cerevisiae in different growth media. J. Bacteriol. 98, 458–466 (1969)
Wickner, R.B.: Deletion of mitochondrial DNA bypassing a chromosomal gene needed for maintenance of the killer plasmid of yeast. Genetics 87, 441–452 (1977)
Wickner, R.B., Leibowitz, M.J.: Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: The killer character of yeast. J. Mol. Biol. 105, 427–434 (1976)
Wickner, R.B., Leibowitz, M.: Dominant chromosomal mutation bypassing chromosomal genes needed for killer RNA plasmid replication in yeast. Genetics 87, 453–469 (1977)
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Communicated by Ch. Auerbach
Paper III in this series is Elliott and McLaughlin (Molec. Gen. Genet., In press)
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Clare, J.J., Oliver, S.G. The regulation of RNA synthesis in yeast IV. Molec. Gen. Genet. 171, 161–166 (1979). https://doi.org/10.1007/BF00270002
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DOI: https://doi.org/10.1007/BF00270002