Abstract
Root formation and in vivo nitrate reductase (NR) activity were determined in leafy spurge cell suspensions. Cells grown in B5 media with 1 mg L−1 2,4-D were transferred to B5 media without 2,4-D, but containing either high (92:8) or low (15:85) ratios of nitrogen as NO −3 -N:NH +4 -N. In older cell lines root formation occurred only in the low NO −3 medium with =<30 roots per flask. In younger cell lines root numbers were greatest in the high NO −3 medium (1000 to 3000 per flask). Cells grown in low NO −3 medium were about one-third the final dry weight as those in high NO −3 medium. Root length was consistently greater for cell lines of all ages in the low NO −3 medium. Developmental profiles of NR activity were similar in cell lines of all ages, whether or not roots were formed. NR activity was lower, however, in cultures grown in low NO −3 medium compared to high NO −3 medium. There was no consistent relationship between NR activity and root initiation. Therefore, nitrate reductase does not appear to be a primary target for regulation of leafy spurge growth by chemical application.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bonner JA (1965) In: Bonner JA, Varner JE (eds) Plant Biochemistry. Academic Press, New York, pp 850–866
Caldas RA, Caldas LS (1976) Physiol Plant 37: 111–116
Christianson ML, Warnick DA, Carlson PS (1983) Science 222: 632–634
Christianson ML, Warnick DA (1985) Dev Biol 112: 494–497
Davis DG, Olson PA, Stolzenberg RA (1988) Plant Cell Reports in press
Dougall DK (1980) In: Staba EJ (ed) Plant Tissue Culture as a Source of Biochemicals. Chemical Rubber Company Press, Boca Raton, FL, pp 21–58
Dwivedi UN, Khan BM, Rawal SK, Mascarenhas AF (1984) J Plant Physiol 117: 7–15
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158
Gamborg, OL (1970) Plant Physiol 45: 372–375
Hahlbrock K (1974) In: Street HE (ed) Tissue Culture and Plant Science. Academic Press, London, pp 363–378
Hamill JD, Cocking EC (1986) J Plant Physiol 123: 289–298
Hunault G (1986) Ann Sci Nat Bot Biol Veg 7(2): 63–76
Jones RW, Abbot AJ, Hewitt EJ, James DM, Best GR (1976) Planta 133: 27–34
Jordan DB, Fletcher JS (1980) Can J Bot 58: 1088–1094
Kadam SS, Johari RJ, Ramarao CS, Srinivasan (1980) Phytochem 19: 2095–2097
Kamada H, Harada, H (1984) Biochem Physiol Pflanzen 179: 403–410
Martin SM, Rose D (1976) Can J Bot 54: 1264–1270
Marton L, Dung TM, Mendel RR, Maliga P (1982) Mol Gen Genet 182: 301–304
Masuda K, Kikuta Y, Okazawa Y (1981) Jap J Crop Sci 50(2): 125–130
Sims AP, Folkes BF, Bussey AH (1968) In: Hewitt EJ, Cutting CV (eds) Recent Advances in Nitrogen Metabolism in Plants. Academic Press, NY, pp 91–114
Skirvin RM, Chu MC, Mann ML, Young H, Sullivan J, Fermanian T (1986) Plant Cell Reports 5: 292–294
Thomas E, Street HE (1972) Ann Bot 36: 239–247
Thorpe TA (1980) Int Rev Cytol Suppl 11A: 71–111
Thorpe TA (1982) In: Thomas DT, Ellis BE, Harney PM, Kasha KJ, Peterson RL (eds) Application of Plant Cell and Tissue Culture to Agriculture and Industry. Univ. Guelph, Guelph, Ont., pp 115–138
Wetherell DF, Dougall DK (1976) Physiol Plant 37: 97–103
White DWR (1984) Planta 162: 1–7
Yasuda T, Hasegawa PM, Cheng T-Y (1980) Physiol Plant 48: 83–87
Author information
Authors and Affiliations
Additional information
Communicated by J. M. Widholm
Rights and permissions
About this article
Cite this article
Evenson, K.J., Galitz, D.S. & Davis, D.G. The relationship of nitrogen source and in vivo nitrate reductase actiity to root formation in Euphorbia esula cell suspension cultures. Plant Cell Reports 7, 361–364 (1988). https://doi.org/10.1007/BF00269938
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269938