Skip to main content

A new protocol for isolation of mitochondrial DNA from cotton seedlings

Plant Cell Reports volume 7, pages 356–360 (1988)Cite this article

Abstract

A procedure to isolate mtDNA from cotton seedlings G. hirsutum and G. barbadense has been developed. The new protocol allows for the isolation of cotton mtDNA of high purity, yield and digestibility by restriction endonucleases. The success of the protocol is based on critical adjustments in the ionic strength of the homogenizing medium and washing buffer, the speed of grinding during homogenization, and the methods used for lysis of the mitochondria.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Brennicke, A. (1980). Mitochondrial DNA from Oenothera berteriana. Plant Physiology 65: 1207–1210.

    Google Scholar 

  • DeBonte, L. R., B. F. Matthews and K. G. Wilson. (1984). Variation of plastid and mitochondrial DNAs in the genus Daucus. Amer. J. Bot. 71: 932–940.

    Google Scholar 

  • deHass, J. M. and A. J. Kool. (1982). Isolation of mitochondrial DNA from petunia hybrida suspension culture cells. PMB Newsletter 3: 3–5.

    Google Scholar 

  • Hanson, M. R., M. L. Boeshore, P. E. McClean, M. A. O'Connell and H. T. Nivision. (1986). The isolation of mitochondria and mitochondrial DNA. Methods in Enzymology 118: 437–453.

    Google Scholar 

  • Maga, J. A. and K. Lorenz. (1974). Gas-liquid chromatography separation of the free phenolic acid fractions in various oilseed protein sources. J. Sci. Food Agri. 25: 797–802.

    Google Scholar 

  • Maniatis, T., E. F. Fritsch and J. Sambrok. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY.

  • Moore, A. L. and M. O. Proudlove. (1983). Mitochondria and sub-mitochondrial particles: In isolation of membrane and organelles from plant cells. Eds. J. L. Hall and A. L. Moore, pp. 153–183, Academic Press, London.

    Google Scholar 

  • Pring, D. R. and C. S. Levings, III. (1978). Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89: 121–136.

    Google Scholar 

  • Sappenfield, W. P. and R. H. Dilday. (1980). Breeding high terpenoid cottons the 1978 regional tests. Proc. Beltwide Cotton Prod. Res. Conf., pp. 92–93.

Download references

Author information

Affiliations

  1. Plant Physiology and Genetics Program, The University of Tennessee, 37996, Knoxville, TN, USA

    C. L. Hsu

  2. Department of Botany and Plant Physiology and Genetics Program, The University of Tennessee, 37996, Knoxville, TN, USA

    B. C. Mullin

Authors
  1. C. L. Hsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. C. Mullin
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by L. K. Grill

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hsu, C.L., Mullin, B.C. A new protocol for isolation of mitochondrial DNA from cotton seedlings. Plant Cell Reports 7, 356–360 (1988). https://doi.org/10.1007/BF00269937

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269937

Key words

  • Cotton seedlings
  • Mitochondrial DNA
  • isolation protocol