Abstract
Protoplasts were isolated from immature cotyledons of six cultivars of Glycine max L. and cultured in the KP8 liquid medium supplemented with 0.2 mg/L 2,4-D, 1 mg/L NAA and 0.5 mg/L ZT. The protoplasts started to divide after 3–5 days of culture. Sustained divisions resulted in mass production of cell colonies and small calli in 6 weeks. The calli further grew to 2–3 mm on the gelritesolidified K8 medium and were transferred onto the MSB medium with 1 mg/L 2,4-D and 0.25 mg/L BA, to obtain compact and nodular calli. Shoot formation was initiated on MSB medium with 0.15 mg/L NAA, and BA, KT and ZT, 0.5 mg/L of each, with or without 500 mg/L CH. It was followed by plant regeneration. So far, 87 plants have been regenerated from 4 cultivars, and normal seeds were obtained from them after transplanting into pots.
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Abbreviations
- IAA:
-
indol-3-acetic acid
- NAA:
-
naphthalene acetic acid
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- KT:
-
kinetin
- BA:
-
6-benzyladenine
- ZT:
-
zeatin
- CH:
-
casein hydrolysate
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Communicated by I. K. Vasil
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Wei, Zm., Xu, Zh. Plant regeneration from protoplasts of soybean (Glycine max L.). Plant Cell Reports 7, 348–351 (1988). https://doi.org/10.1007/BF00269935
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DOI: https://doi.org/10.1007/BF00269935