Abstract
Plants were regenerated by somatic embryogenesis from endosperm tissue of open-pollinated seeds of Juglans regia L. cv Manregian. These plants were obtained by growing endosperm tissue on media similar to those used for plant regeneration from walnut cotyledons (Tulecke and McGranahan 1985). The plants appear morphologically uniform and have a triploid chromosome number of 3n=48. Nine plants have been grown to a young sapling stage in soil. This embryogenic line from endosperm has been maintained in culture for two years by the process of repetitive somatic embryogenesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ball EA, Morris DM, Rydelius JA (1978) Belgiques pp 181–228.
Bhatnagar SP, Sawhney V (1981) Int Rev Cytol 73:55–102.
Bradley GL (1982) Giemsa-band karyotyping and the cytogenetics and evolution of Fragaria species, PhD Diss University of California, Davis.
Cheema GS, Mehra PN (1982) Morphogenesis in endosperm cultures. Proc 5th Intl Cong Plant Tissue and Cell Culture, Tokyo, Japan.
Driver JA, Kuniyuki AH (1984) HortScience 19:507–509.
Gibson MD (1964) The Manregian walnut, Northern Nut Growers Assoc 55:105–109.
Huang Z, Huangfu Y, Xu L (1983) Kexue Tongbao 28:112–117.
Johri BM, Bhojwani SS (1977) In: Reinert J, Bajaj YPS (eds) Plant Cell Tissue and Organ Culture, Springer Verlag pp 398–411.
Lakshmi Sita G (1987) In: Bonga JM, Durzan DJ (eds) Cell and Tissue Culture in Forestry, Martinus Nijhoff/Dr W Junk, The Netherlands, pp 269–284.
Lakshmi Sita G, Raghavan NV, Ram R, Vaidyanathan CS (1980) Plant Sci Lett 20:63–69.
McGranahan GH, Catlin PB (1987) In: Rom RC, Carlson RF (eds) Rootstocks for Fruit Crops, John Wiley & Sons, New York pp 411–450.
McGranahan GH, Tulecke W, Arulsekar S, Hansen JJ (1986) J Soc Hort Sci 111:627–630.
McGranahan, GH, Driver JA, Tulecke W (1987) In: Bonga JM, Durzan DJ (eds) Cell and Tissue Culture in Forestry, Martinus Nijhoff/Dr W Junk, The Netherlands, pp 261–271.
Mu SK, Liu SQ, Zhou YK, Qian NF, Zhang P, Cie HX, Zhang FS, Yan ZL (1977) Scientia Sinica 20:370–376.
Muniyamma A (1977) Ann Bot 41:1077–1079.
Srivastava PS (1973) Z Pflanzenphysiol 69:270–273.
Tulecke W, McGranahan G (1985) Plant Sci 40:53–67.
Wepster BM (1970) Manregian walnuts, Northern Nut Growers Assoc 61:36–40.
Author information
Authors and Affiliations
Additional information
Communicated by I. K. Vasil
Rights and permissions
About this article
Cite this article
Tulecke, W., McGranahan, G. & Ahmadi, H. Regeneration by somatic embryogenesis of triploid plants from endosperm of walnut, Juglans regia L. cv Manregian. Plant Cell Reports 7, 301–304 (1988). https://doi.org/10.1007/BF00269923
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269923