Summary
The selection at 22° C of yeast cordycepin (3′-deoxyadenosine) sensitive mutants which would be temperature-sensitive at 37°C allowed the obtention of mutants specifically impaired in processing of Poly(A)-containing RNAs at 37°C. The mutants displaying this phenotype belong to two different loci. The biochemical study of the physiological function which is blocked by the mutation has revealed that the level of radioactive Poly(A)-containing RNAs found in a 5 min pulse after a 10 min shift at 37°C is 6 times less in the mutants than in the wild type without reduction of the non Poly(A)-containing RNAs fraction. Further studies have shown no alteration in the two Poly(A) polymerases activities and suggest strongly a faster decay of Poly(A)-containing RNAs in the mutants.
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Communicated by F.Kaudewitz
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Bloch, J.C., Perrin, F. & Lacroute, F. Yeast temperature-sensitive mutants specifically impaired in processing of Poly(A)-Containing RNAs. Molec. Gen. Genet. 165, 123–127 (1978). https://doi.org/10.1007/BF00269900
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DOI: https://doi.org/10.1007/BF00269900