Skip to main content
SpringerLink
Log in

pet 18: A chromosomal gene required for cell growth and for the maintenance of mitochondrial DNA and the killer plasmid of yeast

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Mutations in the pet18 gene of Saccharomyces cerevisiae (formerly denoted pets) confer three phenotypes on mutant strains: (i) inability to respire (petite), (ii) inability to maintain the double-stranded RNA killer plasmid (sensitive), and (iii) temperature sensitivity for growth. We find that pet18 mutants lack mitochondrial DNA. However, despite their inability to maintain the killer RNA plasmid and mitochondrial DNA, pet18 mutants still can carry the other yeast plasmids, [URE3-1], [PSI], and 2-micron DNA. The temperature sensitivity of the pet18 mutants is not expressed as a selective defect in total DNA, RNA, or protein synthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aigle, M., Lacroute, F.: Genetical aspects of [URE3], a non-mitochondrial, cytoplasmically inherited mutation in yeast. Molec. gen. Genet. 136, 327–335 (1975)

    Google Scholar 

  • Bevan, E.A., Somers, J., Theivendirarajah, K.: Genes controlling the expression of the killer character in yeast (Saccharomyces cerevisiae) (Abstract). XI Internat. Botanical Congress, p. 14 (1969)

  • Bussey, H.: Effects of yeast killer factor on sensitive cells. Nature (Lond.) New Biol. 235, 73–75 (1972)

    Google Scholar 

  • Chen, S., Ephrussi, B., Hottinguer, H.: Nature genetique des mutants a deficience respiratoire de la souche B-II de la levure de boulangerie. Heredity 4, 337–351 (1950)

    Google Scholar 

  • Cox, B.S.: ψ, A cytoplasmic suppressor of super-suppressor in yeast. Heredity 20, 505–521 (1965)

    Google Scholar 

  • Cox B.S.: A recessive lethal super-suppressor mutation in yeast and other ψ phenomena. Heredity 26, 211–232 (1971)

    Google Scholar 

  • Culbertson, M.R., Charnas, L., Johnson, M.T., Fink, G.R.: Frameshifts and frameshift suppressors in Saccharomyces cerevisiae. Genetics 86, 745–764 (1977)

    Google Scholar 

  • Drillien, R., Aigle, M., Lacroute, F.: Yeast mutants pleiotropically impaired in the regulation of the two glutamate dehydrogenases. Biochem. biophys. Res. Commun. 53, 367–372 (1973)

    Google Scholar 

  • Ephrussi, B., Grandchamp, S.: Etudes sur la suppressivite des mutants a deficience respiratoire de la levure. I. Existence au niveau cellulaire de divers “degres de suppressivite”. Heredity 20, 1–7 (1965)

    Google Scholar 

  • Ephrussi, B., de Margerie-Hottinguer, H., Roman, H.: Suppressiveness: A new factor in the genetic determinism of the synthesis of respiratory enzymes in yeast. Proc. nat. Acad. Sci. (Wash.) 41, 1065–1071 (1955)

    Google Scholar 

  • Fink, G.R., Styles, C.A.: Curing of a killer factor in Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 69, 2846–2849 (1972)

    Google Scholar 

  • Goldring, E.S., Grossman, L.I., Krupnick, D., Cryer, D.R., Marmur, J.: The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J. molec. Biol. 52, 323–335 (1970)

    Google Scholar 

  • Grossman, L.I., Goldring, E.S., Marmur, J.: Preferential synthesis of yeast mitochondrial DNA in the absence of protein synthesis. J. molec. Biol. 46, 367–376 (1969)

    Google Scholar 

  • Haber, J.E.: Genetic control of a sporulation-specific ribosomal RNA in yeast (Abstract). Fed. Proc. 35, 1510 (1967)

    Google Scholar 

  • Hartwell, L.H.: Macromolecule synthesis in temperature-sensitive mutants of yeast. J. Bact. 93, 1662–1670 (1967)

    Google Scholar 

  • Herring, A.J., Bevan, E.A.: Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. J. gen. Virol. 22, 387–394 (1974)

    Google Scholar 

  • Hollenberg, C.P., Borst, P., Thuring, R.W.J., Bruggen, E.F.J. van: Size, structure, and genetic complexity of yeast mitochondrial DNA. Biochim. biophys. Acta (Amst.) 186, 417–419 (1969)

    Google Scholar 

  • Hollenberg, C.P., Degelmann, A., Kustermann-Kuhn, B., Royer, H.D.: Characterization of 2-μm DNA of Saccharomyces cerevisiae by restriction fragment analysis and integration in an Escherichia coli plasmid. Proc. nat. Acad. Sci. (Wash.) 73, 2072–2076 (1976)

    Google Scholar 

  • Lacroute, F.: Non-Mendelian mutation allowing ureidosuccinic acid uptake in yeast. J. Bact. 106, 519–522 (1971)

    Google Scholar 

  • Leibowitz, M.J., Wickner, R.B.: A chromosomal gene required for killer plasmid expression, mating, and spore maturation in Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.), 73, 2061–2065 (1976)

    Google Scholar 

  • Liebman, S.W., Stewart, J.W., Sherman F.: Serine substitutions caused by an ochre suppressor in yeast. J. molec. Biol. 94, 595–610 (1975)

    Google Scholar 

  • Livingston, D.M.: Inheritance of the 2 μm DNA plasmid from Saccharomyces. Genetics 86, 73–84 (1977)

    Google Scholar 

  • Lloyd, D.: The mitochondria of microorganisms. London: Academic Press 1974

    Google Scholar 

  • Mackler, B., Douglas, H.C., Will S., Hawthorne D.C., Mahler, H.R.: Biochemical correlates of respiratory deficiency. IV. Composition and properties of respiratory particles from mutant yeasts. Biochemistry 4, 2016–2020 (1965)

    Google Scholar 

  • Michaelis, G., Douglass, S., Tsai, M., Criddle, R.S.: Mitochondrial DNA and suppressiveness of petite mutants in Saccharomyces cerevisiae. Biochem. Genet. 5, 487–494 (1971)

    Google Scholar 

  • Mortimer, R.K., Hawthorne, D.C.: Genetic mapping in Saccharomyces. IV. Mapping of temperature-sensitive genes and use of disomic strains in localizing genes. Genetics 74, 33–54 (1973)

    Google Scholar 

  • Mortimer, R.K., Hawthorne, D.C.: Genetic mapping in yeast. In: Methods in cell biology. (D.M., Prescott, ed.), pp. 221–233, Vol. 11. New York: Academic Press 1975

    Google Scholar 

  • Nagley, P., Linnane, A.W.: Mitochondrial DNA deficient petite mutants of yeast. Biochem. biophys. Res. Commun. 39, 989–996 (1970)

    Google Scholar 

  • Newlon, C.S., Fangman, W.L.: Mitochondrial DNA synthesis in cell cycle mutants of Saccharomyces cerevisiae. Cell 5, 423–428 (1975)

    Google Scholar 

  • Ogur, M., Roshanmanesh, A., Ogur, S.: Tricarboxylic acid cycle mutants in Saccharomyces: Comparison of independently derived mutants. Science 147, 1590 (1965)

    Google Scholar 

  • Robinow, C.F.: The preparation of yeasts for light microscopy. In: Methods in cell biology. (D.M. Prescott, ed.), pp 1–22, Vol. 11. New York: Academic Press 1975

    Google Scholar 

  • Schatz, G., Haslbrunner, E., Tuppy, H.: Deoxyribonucleic acid associated with yeast mitochondria. Biochem. biophys. Res. Commun. 15, 127–132 (1964)

    Google Scholar 

  • Sherman, F.: The effects of elevated temperatures on yeast. II. Induction of respiratory-deficient mutants. J. cell. comp. Physiol. 54, 37–52 (1959)

    Google Scholar 

  • Sherman, F.: Respiration-deficient mutants of yeast. I. Genetics. Genetics 48, 375–385 (1963)

    Google Scholar 

  • Sinclair, J.H., Stevens, B.J., Songhavi, P., Rabinowitz, M.: Mitochondrial satellite and circular DNA filaments in yeast. Science 156, 1234–1237 (1967)

    Google Scholar 

  • Somers, J.M., Bevan, E.A.: The inheritance of the killer character in yeast. Genet. Res. 13, 71–83 (1969)

    Google Scholar 

  • Terawi, K.K., Jayaraman, J., Mahler, H.R.: Separation and characterization of mitochondrial DNA from yeast. Biochem. biophys. Res. Commun. 21, 141–148 (1965)

    Google Scholar 

  • Vodkin, M., Katterman, F., Fink, G.R.: Yeast killer mutants with altered double-stranded reibonucleic acid. J. Bact. 117, 681–686 (1974)

    Google Scholar 

  • Wickner, R.B.: “Killer character” of Saccharomyces cerevisiae: curing by growth at elevated temperature. J. Bact. 117, 1356–1357 (1974a)

    Google Scholar 

  • Wickner, R.B.: Chromosomal and nonchromosomal mutations affecting the “killer character” of Saccharomyces cerevisiae. Genetics 76, 423–432 (1974b)

    Google Scholar 

  • Wickner, R.B.: Killer of Saccharomyces cerevisiae: a doublestranded ribonucleic acid plasmid. Bact. Rev. 40, 757–773 (1976)

    Google Scholar 

  • Wickner, R.B.: Deletion of mitochondrial DNA bypassing a chromosomal gene needed for maintenance of the killer plasmid of yeast. Genetics 87, 441–452 (1977)

    Google Scholar 

  • Wickner, R.B., Leibowitz, M.J.: Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast. J. molec. Biol. 105, 427–443 (1976)

    Google Scholar 

  • Wickner R.B., Leibowit M.J.: Dominant chromosomal mutation bypassing chromosomal genes needed for killer RNA plasmid replication in yeast. Genetics 87, 453–469 (1977)

    Google Scholar 

  • Woods, D.R., Bevan, E.A.: Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J. gen. Microbiol. 51, 115–126 (1968)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, College of Medicine and Dentistry of New Jersey, Rutgers Medical School, 08854, Piscataway, New Jersey

    Michael J. Leibowitz

  2. Laboratory of Biochemical Pharmacology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, 20014, Bethesda, Maryland

    Michael J. Leibowitz & Reed B. Wickner

Authors
  1. Michael J. Leibowitz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Reed B. Wickner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Leibowitz, M.J., Wickner, R.B. pet 18: A chromosomal gene required for cell growth and for the maintenance of mitochondrial DNA and the killer plasmid of yeast. Molec. Gen. Genet. 165, 115–121 (1978). https://doi.org/10.1007/BF00269899

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269899

Keywords

Search

Navigation