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Post-transcriptional regulation of methionine content in maize kernels

Molecular and General Genetics MGG volume 225, pages 331–339 (1991)Cite this article

Summary

Message levels for a methionine-rich 10 kDa zein were determined in three inbred lines of maize and their reciprocal crosses at various stages during endosperm development. Inbred line BSSS-53, which overexpresses the 10 kDa protein in mature kernels, was shown to have higher mRNA levels in developing endosperm, as compared to inbred lines W23 and W64A. Differences in mRNA levels could not be explained by differences in transcription rate of the 10 kDa zein gene, indicating differential post-transcriptional regulation of this storage protein in the different inbred lines analyzed. Among progeny segregating for the BSSS-53 allele of the 10 kDa zein structural gene Zps10l(22), mRNA levels are independent of Zps10/(22) segregation, indicating that post-transcriptional regulation of mRNA levels takes place via a trans-acting mechanism. In the same progeny, mRNA levels are also independent of allelic segregation of the regulatory locus Zpr10/(22). Thus, the trans-acting factor encoded by Zpr10/(22) determines accumulation of 10 kDa zein at a translational or post-translational step. Multiple trans-acting factors are therefore involved in post-transcriptional regulation of the methionine-rich 10 kDa zein.

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Author notes

  1. Julie A. Kirihara

    Present address: Plant Science Research Inc., 10320 Bren Road East, 55343, Minnetonka, PC, USA

Affiliations

  1. Waksman Institute, Rutgers, The State University of New Jersey, 08855, Piscataway, NJ, USA

    Marilyn Cruz-Alvarez, Julie A. Kirihara & Joachim Messing

Authors
  1. Marilyn Cruz-Alvarez
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  2. Julie A. Kirihara
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  3. Joachim Messing
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Communicated by H. Saedler

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Cruz-Alvarez, M., Kirihara, J.A. & Messing, J. Post-transcriptional regulation of methionine content in maize kernels. Molec. Gen. Genet. 225, 331–339 (1991). https://doi.org/10.1007/BF00269866

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  • DOI: https://doi.org/10.1007/BF00269866

Key words

  • Maize
  • Storage protein
  • Run-on transcription
  • Post-transcriptional regulation
  • Trans-acting factors