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Alkaloid accumulation in Ca-alginate entrapped cells of Catharanthus roseus: Using a limiting growth medium

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Abstract

The limitation of growth of Catharanthus roseus cells was investigated with a view to their entrapment in a Ca-alginate matrix. An examination of the effects of lowered 2,4-D and phosphate concentrations on cell viability and indole alkaloid biosynthesis enabled a growth limiting and product formation stimulating medium to be designed. Entrapped cells showed a retention of both respiratory activity and biosynthetic capacity over an extended period of time compared with free cells. Evidence is presented which suggests that immobilization in Ca-alginate beads acts to stabilize cells, resulting in enhanced product accumulation.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

d.w.:

dry weight

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Authors and Affiliations

  1. Département de Génie Biochimique et Alimentaire, UA-CNRS-No 544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France

    Florence Majerus & Alain Pareilleux

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  1. Florence Majerus
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  2. Alain Pareilleux
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Communicated by A.M. Boudet

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Majerus, F., Pareilleux, A. Alkaloid accumulation in Ca-alginate entrapped cells of Catharanthus roseus: Using a limiting growth medium. Plant Cell Reports 5, 302–305 (1986). https://doi.org/10.1007/BF00269828

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  • DOI: https://doi.org/10.1007/BF00269828

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