Abstract
In vitro regeneration of plants from both cotyledon-, and leaf — derived calli of Lotononis bainesii Paker was studied under defined nutritional, hormonal and environmental conditions. Explants from both, cotyledons from seedilings of 4 days old and fully expanded leaves from mature plants, were cultured on MS medium containing 0.8% agar and supplemented with 0.01, 0.1, and 1 mg/1 concentrations of naphthaleneacetic acid (NAA) and 0.1, 1, and 3 mg/1 levels of benzyladenine (BA) in various combinations. Multiple shoot (on an average 4 shoots per callus) regeneration from primary callus occurred within 15 to 35 days of culture in most of the media tested. Although the best medium for shoot regeneration from cotyledon-derived callus contained NAA and BA at 1, and 0.1 mg/1 levels, respectively, maximal shoot regeneration from leaf-derived calli was achieved by using NAA and BA at 0.01 and 0.1 mg/1, respectively. Roots were induced to differentiate by transferring the regenerated shoots onto a medium lacking growth regulators.
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Mroginski LA, Kartha KK (1984) In: Janick J (ed.) Plant Breeding Reviews, vol. 2, AVI Publ. Co, Westport, CT. USA, pp 215–264
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497
'tMannetje L, O'Connor KF, Burt RL (1980) In: Summerfield RJ, Bunting AH (eds.) Advances in Legume Science, Royal Botanic Gardens, Kew, England, pp 537–551
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Communicated by F. Constabel
Supported by a research fellowship from Consejo Nacional de Investigaciones Científicas y Técnicas (República Argentina).
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Bovo, O.A., Mroginski, L.A. & Rey, H.Y. Regeneration of plants from callus tissue of the pasture legume Lotononis bainesii . Plant Cell Reports 5, 295–297 (1986). https://doi.org/10.1007/BF00269826
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DOI: https://doi.org/10.1007/BF00269826