Skip to main content
Log in

Techniques for enhanced release of leaf protoplasts in Populus

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Microscopic examination of Populus leaf tissue following enzyme treatment revealed two factors contributing to low protoplast yields: (1) poor penetration of the enzymes into the tissue, and (2) entrapment of protoplasts in leaf debris during protoplast purification procedures. A simple combination of rapid grinding of the tissue in an Omni-mixer prior to enzyme treatment and forceful washing of leaf-debris after digestion provided high exposure of the cells, uniform digestion, and high yields of protoplasts of two Populus clones. Protoplasts exhibited cell wall regeneration and long-term viability in culture. The relative yield advantages of the techniques varied with the inherent digestibility of each clone but could produce up to 600 percent greater protoplast yields in a woody plant species in which protoplast isolation was previously limited. The techniques were also applicable to an herbaceous species, Solanum etuberosum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

BA:

benzyladenine

NAA:

naphthalene acetic acid

WPM:

Woody Plant Medium, Lloyd and McCown (1980)

MS:

Murashige and Skoog Medium (1962)

(NC-XXXX):

North Central Forest Experiment Station accession number assigned to Populus hybrid clones

References

  • Ahuja MR (1983) Silvae Genetica 32:225–227

    Google Scholar 

  • Ahuja MR (1984) Silvae Genetica 33:32–37

    Google Scholar 

  • Butt AD (1985) Plant Sci 42:55–59

    Google Scholar 

  • Evans DA, Bravo JE (1983) In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of Plant Cell Culture, vol I, MacMillan Publ. Co., New York, pp. 124–176

    Google Scholar 

  • Haberlach GT, Cohen BA, Reichert NA, Baer MA, Towill LE, Helgeson JP (1985) Plant Sci 39:67–74

    Google Scholar 

  • Lloyd GB, McCown BH (1980) Proc Intl Plant Prop Soc 30:421–427

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    Google Scholar 

  • Patniak G, Wilson D, Cocking EC (1981) Z Pflanzenphysiol 102:199–205

    Google Scholar 

  • Russell JA, McCown BH (1986) submitted

  • Schenk RU, Hildebrandt AC (1969) Crop Sci 9:629–631

    Google Scholar 

  • Smith MAL (1984) PhD Thesis, University of Wisconsin, Madison, Wisconsin p105

  • Smith MAL, McCown BH (1982/83) Plant Sci Lett 28:149–156

    Google Scholar 

  • Verma DC, Wann SH (1983) In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) 6th Intl Protoplast Symp, Basel, Experentia Suppl 45:10

  • Widholm JM (1972) Stain Technology 47:189–194

    Google Scholar 

  • Wright DC (1985) Plant Cell Tissue Organ Culture 4:95–100

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by G. C. Phillips

Rights and permissions

Reprints and permissions

About this article

Cite this article

Russell, J.A., McCown, B.H. Techniques for enhanced release of leaf protoplasts in Populus . Plant Cell Reports 5, 284–287 (1986). https://doi.org/10.1007/BF00269823

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269823

Keywords

Navigation