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Examination of genome stability in cultured Lycopersicon

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Abstract

The genetic stability of plants regenerated from either mesophyll protoplasts or leaf slices of the F1 hybrid between Lycopersicon esculentum and L. pennellii was assayed by comparing the ploidy level, leaf morphology and isozyme patterns of the regenerants with their somatic parents. Regenerants from protoplasts were predominantly tetraploid, regenerants from leaf slices were predominantly diploid; both classes of regenerants had isozyme patterns identical to those of the parent plant. Callus was analyzed that grew up from cultures containing fused protoplasts from either irradiated or untreated protoplasts of L. esculentum and L. pennellii. The L. pennellii cell line used was 18 months old and could no longer regenerate. Out of 75 calli scored at 3 isozyme loci, 51 were heterozygous at only one or two of the loci. Irradiation of the two parental lines was not necessary to produce fusion products exhibiting asymmetric expression of parental genes.

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Abbreviations

Got-2:

glutamate oxaloacetate transaminase-2

Pgi-1:

phosphoglucoisomerase-1

Pgm-2:

phosphoglucomutase-2

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Author notes

  1. Mary A. O'Connell

    Present address: Plant Genetic Engineering Laboratory and Department of Horticulture, New Mexico State University, 88003, Las Cruces, NM, USA

  2. Maureen R. Hanson

    Present address: Section of Genetics and Development, Bradfield Hall, Cornell University, 14853, Ithaca, NY, USA

Authors and Affiliations

  1. Department of Biology, University of Virginia, 22901, Charlottesville, VA, USA

    Mary A. O'Connell, Linda P. Hosticka & Maureen R. Hanson

Authors
  1. Mary A. O'Connell
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  2. Linda P. Hosticka
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  3. Maureen R. Hanson
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Communicated by G. C. Phillips

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O'Connell, M.A., Hosticka, L.P. & Hanson, M.R. Examination of genome stability in cultured Lycopersicon . Plant Cell Reports 5, 276–279 (1986). https://doi.org/10.1007/BF00269821

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  • DOI: https://doi.org/10.1007/BF00269821

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