Skip to main content
SpringerLink
Log in

Plant regeneration from mesophyll protoplasts in commercial potato cultivars (Primura, Kennebec, Spunta, Desirée)

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

In view of an evaluation of the relative efficiency of different in vitro systems for mutant induction and isolation in potato, a procedure of plant regeneration from protoplasts of some potato (Solanum tuberosum L.) cultivars was developed. Four cultivars (Primura, Kennebec, Spunta, Desirée) were used for isolation, culture and regeneration of leaf mesophyll protoplasts.

These lines were chosen because of their economic importance in Italy and in the case of cv. Desirée for the presence of markers useful in the morphological characterization of regenerants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

MS :

Murashige and Skoog's medium

BAP :

6-benzylaminopurine

2,4 D :

2,4-dichlorophenoxyacetic acid

NAA :

naphthaleneacetic acid

GA 3 :

gibberellic acid

ZT :

zeatin

References

  • Bokelmann G.S. and Roest S. (1983). Z. Pflanzenphysiol. 109: 259–265.

    Google Scholar 

  • Broertjes, C. and A.M. Van Harten (1978) in: Application of mutation breeding in the improvement of vegetatively propagated crops: an interpretative literature review. Elsevier Sci. Publ. Co.

  • Harten A.M. Van (1978) Agric. Res. Rep. 873.DUPOC Wageningen 132 p.

  • Harten A.M. Van, and Bouter H. (1973) Euphytica 22: 1–7.

    Google Scholar 

  • Karp A., Nelson R.S., Thomas E., Bright S.W.J. (1982) Theor. Appl. Genet. 63: 265–272.

    Google Scholar 

  • Larkin, P.J., Scowcroft W.R. (1981): Theor. Appl. Genet. 60: 197–214.

    Google Scholar 

  • Lörz H., Larkin P.J., Thomson J. and Scowcroft W.R. (1983) Plant Cell Tissue Organ Culture 2: 217–226.

    Google Scholar 

  • Murashige T. and Skoog F. (1962). Physiol. Plant. 15: 437–497.

    Google Scholar 

  • Roest S. and G.S. Bokelmann (1983) in: I. Potrykus, C. T. Harms, A. Hinnen, R. Hutter, P.J. King and R. D. Shillito (Eds.), PROTOPLASTS 1983 Post Proc., Birkhauser Verlag Basel - Boston -Stuttgart p. 282–283

    Google Scholar 

  • Shepard J.F. and Totten R.. (1977) Plant Physiol. 60: 313–316.

    Google Scholar 

  • Shepard J.F. (1980) In: Genetic Improvement of Crops: Emergent Techniques 185–189, Minneapolis, Univ. of Minnesota Press.

    Google Scholar 

  • Sonnino A., Ancora G. and Locardi C. (1985) In: International Symposium on Nuclear Techniques and in-vitro culture for plant improvement, IAEA, Wien; p. 385–394.

    Google Scholar 

  • Wenzel, G. (1980) In: Advance in Protoplast Research 327–340 Oxford: Pergamon Press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ENEA, Divisione Tecnologie Biologiche e Agrarie, C.R.E. Casaccia, I-00060, Rome, Italy

    R. Tavazza & G. Ancora

Authors
  1. R. Tavazza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Ancora
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Lörz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tavazza, R., Ancora, G. Plant regeneration from mesophyll protoplasts in commercial potato cultivars (Primura, Kennebec, Spunta, Desirée). Plant Cell Reports 5, 243–246 (1986). https://doi.org/10.1007/BF00269812

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269812

Keywords

Search

Navigation