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Polynucleotide kinase mutant of bacteriophage T4

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Summary

We report the isolation and biological properties of two T4 phage mutants which are deficient in polynucleotide kinase. These two mutants are wild type with respect to replication, genetic recombination and DNA repair (as measured by sensitivity to UV irradiation, γ-irradiation and 32P decay). These negative results suggest that this phage-induced enzyme is not a critical factor in DNA metabolism.

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Authors and Affiliations

  1. Cancer Research Laboratory, University of Western Ontario, London, Ontario, Canada

    Voon Loong Chan (Fellow of the National Cancer Institute of Canada) & Kaney Ebisuzaki

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  1. Voon Loong Chan
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  2. Kaney Ebisuzaki
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Communicated by Jun-Ichi Tomizawa

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Chan, V.L., Ebisuzaki, K. Polynucleotide kinase mutant of bacteriophage T4. Molec. Gen. Genet. 109, 162–168 (1970). https://doi.org/10.1007/BF00269652

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  • DOI: https://doi.org/10.1007/BF00269652

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