Summary
Eleven lac - mutants have been isolated producing β-galactosidase mutant proteins, which can be activated to enzyme activity upon addition of anti β-galactosidase antibodies (lac -aba -mutants). The mutants have been mapped using P1 transduction and deletion mapping. Seven of them fall into one group (1), two others into another group (2). Two mutants map at sites different from the two groups (Fig. 2). Lac -aba -mutant sites farthest apart correspond to a distance of about 3/4 of the z gene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beckwith, J. R.: A deletion analysis of the lac operator region in E. coli. J. molec. Biol. 8, 427 (1964).
Beckwith, J. R. Lac: The genetic system. In: The lactose operon (J. R. Beckwith and D. Zisper, eds.). Cold Spring Harb. Monogr. 1970.
Cinader, B.: Antibodies to biologically active molecules. Oxford: Pergamon Press 1967.
Cohn, M., Torriani, A. M.: Immunochemical studies with the β-galactosidase and structurally related proteins of E. coli. J. Immunol. 69, 471 (1952).
Craven, G., Steers, E., Anfinsen, C. G.: Purification, composition, and molecular weight of the β-galactosidase of E. coli K 12. J. biol. Chem. 240, 2468 (1965).
Fowler, A. V., Zabin, I.: β-Galactosidase: Immunological studies of nonsense, missense and deletion mutants: J. molec. Biol. 33, 35 (1968).
Garen, A., Zinder, N. D.: Radiological evidence for partial genetic homology between bacteriophage and host bacteria. Virology 1, 347 (1955).
Hirota, Y.: The effect of acridine dyes on mating type factors in E. coli. Proc. nat. Acad. Sci. (Wash.) 46, 57 (1960).
Hughes, R. C., Jeanloz, R. W.: The extracellular glycosidases of Diplococcus pneumoniae. Purification and properties of a neuraminidase and a β-galactosidase. Action on the 160-1 glycoprotein of human plasma. Biochemistry 3, 1535 (1964).
Jacob, F., Ullmann, A., Monod, J.: Le promoteur, élément génétique nécessaire à l'expression d'un opéron. C. R. Acad. Sci. (Paris) 258, 3125 (1964).
Langridge, J.: Genetic evidence for the disposition of the substrate binding site of β-galactosidase. Proc. nat. Acad. Sci. (Wash.) 60, 1260 (1968).
Lederberg, S., Rotman, B., Lederberg, V.: Distribution and activity of single β-D-galactosidase centers among ribosomes of E. coli. J. biol. Chem. 239, 54 (1964).
Melchers, F., Messer, W.: Enhanced stability against heat denaturation of E. coli wild type and mutant β-galactosidase in the presence of specific antibodies. Biochem. biophys. Res. Commun. 40, 570 (1970a).
Melchers, F., Messer, W.: The activation of mutant β-galactosidase by specific antibodies. Purification of eleven antibody activatable mutant proteins and their subunits on Sepharose immunosorbents and determination of the molecular weights by sedimentation analysis and acrylamide gel electrophoresis. Europ. J. Biochem. (1970b) (in press).
Messer, W., Melchers, F.: The activation of mutant β-galactosidase by specific antibodies. In: The lactose operon (J. R. Beckwith and D. Zisper, eds.), p. 305. Cold Spring Harb. Monogr. 1970.
— Vielmetter, W.: High resulution colony staining for the detection of bacterial growth requirement mutants using naphthol azo-dye techniques. Biochem. biophys. Res. Commun. 21, 182 (1965).
Newton, W. A., Beckwith, J. R., Zisper, D., Brenner, S.: Nonsense mutants and polarity in the lac-operon of E. coli. J. molec. Biol. 14, 290 (1965).
Rotman, M. B., Celada, F.: Antibody-mediated activation of a defective β-galactosidase extracted from an E. coli mutant. Proc. nat. Acad. Sci. (Wash.) 60, 660 (1968).
Ullmann, A., Jacob, F., Monod, J.: Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the β-galactosidase structural gene of E. coli. J. molec. Biol. 24, 339 (1967).
Wolf, B., Newman, A., Glaser, D. A.: On the origin and direction of replication of the E. coli K 12 chromosome. J. molec. Biol. 32, 611 (1968).
Author information
Authors and Affiliations
Additional information
Communicated by P. Starlinger
Rights and permissions
About this article
Cite this article
Messer, W., Melchers, F. Genetic analysis of mutants producing defective β-galactosidase which can be activated by specific antibodies. Molec. Gen. Genet. 109, 152–161 (1970). https://doi.org/10.1007/BF00269651
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00269651