Summary
It was previously reported that in Escherichia coli the mutation from erythromycin sensitive to resistance accompanied the alteration of one 50s ribosomal protein component, 50-8, and that the chemical nature of this alteration differed in one mutant to another. In the present paper, it has been demonstrated that the character of the altered 50-8 component was always cotransduced with the erythromycin resistance from the donor to the recipient bacteria by Plkc phage. The geneic locus of these mutations has been shown to be in the str region by transduction and conjugation experiments.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Apirion, D.: Altered ribosomes in a suppressor strain of Escherichia coli. J. molec. Biol. 16, 285–301 (1966).
— Three genes that affect Escherichia coli ribosomes. J. molec. Biol. 30, 255–275 (1967).
Bollen, A., Davies, J., Ozaki, M., Mizushima, S.: Ribosomal protein conferring sensitivity to the antibiotic spectinomycin in Eschrrichia coli. Science 165, 85–86 (1969).
Dekio, S., Takata, R.: Genetic studies of the ribosomal proteins in Escherichia coli II. Altered 30s ribosomal protein component specific to spectinomycin resistant mutants. Molec. Gen. Genetics 105, 219–224 (1969).
Krembel, J., Apirion, D.: Changes in ribosomal proteins associated with mutants in a locus that affects Escherichia coli ribosomes. J. molec. Biol. 33, 363–368 (1968).
Leboy, P. S., Cox, E. C., Flaks, J. G.: The chromosomal site specifying a ribosomal protein in Escherichia coli. Proc. nat. Acad. Sci. (Wash.). 52, 1367–1374 (1964).
Lederberg, J.: Isolation and characterization of biochemical mutants of bacteria. Meth. med. Res. 3, 5–22 (1950).
Mayuga, C., Meier, D., Wang, T.: Escherichia coli: the K12 protein and the streptomycin region of the chromosome. Biochem. biophys. Res. Commun. 33, 203–206 (1968).
O'Neil, D. M., Baron, L.S., Sypherd, P. S.: Chromosomal location of ribosomal protein cistrons determined by intergeneric bacterial mating. J. Bact. 99, 242–247 (1969).
Otaka, E., Itoh, T., Osawa, S.: Ribosomal proteins of bacterial cells: strain and apecies-specificity. J. molec. Biol. 33, 93–107 (1968).
— Teraoka, H., Tamaki, M., Tanaka, K., Osawa, S.: Ribosomes from erythromycin resistant mutants of Escherichia coli Q13. J. molec. Biol. 48, 499–510 (1970).
Rosset, R., Gorini, L.: A ribosomal ambiguity mutation. J. molec. Biol. 39, 95–112 (1969).
Takata, R., Dekio, S., Otaka, E., Osawa, S.: Genetic studies of the ribosomal proteins in Escherichia coli I. Mutants and strains having 30s ribosomal subunit with altered protein components. Molec. Gen. Genetics 105, 113–121 (1969).
Tanaka, K., Teraoka, H., Tamaki, M., Otaka, E., Osawa, S.: Erythromycin-resistant mutant of Escherichia coli with altered ribosomal protein component. Science 162, 576–578 (1968).
Tanaka, Y., Kaji, H.: The role of ribosomal protein for the binding of dihydrostreptomycin to ribosomes. Biochem. biophys. Res. Commun. 32, 313–319 (1968).
Taylor, A. L., Trotter, C. D.: Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353 (1967).
Teraoka, H.: A reversible change in the ability of Escherichia coli ribosomes to bind to erythromycin. J. molec. Biol. 48, 511–513 (1970).
Traub, P., Nomura, M.: Streptomycin resistance mutation in Escherichia coli: altered ribosomal protein. Science 160, 198–199 (1968).
Author information
Authors and Affiliations
Additional information
Communicated by H. G. Wittmann
Rights and permissions
About this article
Cite this article
Takata, R., Osawa, S., Tanaka, K. et al. Genetic studies of the ribosomal proteins in Escherichia coli . Molec. Gen. Genet. 109, 123–130 (1970). https://doi.org/10.1007/BF00269648
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00269648