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Lectin levels in tissues of cultured immature wheat embryos

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Abstract

Levels of wheat germ agglutinin have been determined by radioimmunoassay in tissues of immature wheat embryos cultured under different conditions in order to determine the suitability of the lectin as a marker for somatic embryogenesis. Embryos cultured on media favouring continued embryo development accumulated lectin in a similar manner to zygotic embryos in planta unless precocious germination occurred. Embryos cultured on media containing 2,4-D produced callus, and some of this developed somatic embryos. Both embryogenic and non-embryogenic callus contained WGA, that in non-embryogenic callus possibly arising from developmentally arrested root primordia.

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Abbreviations

ABA:

abscisic acid

dpa:

days post anthesis

PBS:

phosphate buffered saline, (10 mM KH2PO4 K2HPO4, 145 mM NaCl, pH 7.4)

RIA:

radioimmunoassay

WGA:

wheat germ agglutinin

2,4-D:

2,4-dichlorophenoxyacetic acid

References

  • Bright, S.W.J., Jones, M.G.K. (1985) (Eds) Cereal tissue and cell culture. Martinus Nijhoff/Dr. W. Junk, Dordrecht, 303 pp.

    Google Scholar 

  • Crouch, M.L. (1982) Planta 156, 520–524.

    Google Scholar 

  • Haccius, B. (1978) Phytomorphology 28, 74–81.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) J. Biol. Chem. 193, 249–255.

    Google Scholar 

  • Maddock, S.E. (1983) Experimentia Supplementum 45, 14–15.

    Google Scholar 

  • Maddock, S.E., Lancaster, V. A., Risiott, R., Franklin, J. (1983) J. Ex. Bot. 34, 915–926.

    Google Scholar 

  • Mishkind, M.L., Keegstra, K., Palevitz, B.A. (1980) Plant Physiology 66, 950–955.

    Google Scholar 

  • Mishkind, M.L., Raikhel, N.V., Palevitz, B.A., Keegstra, K. (1982) J. Cell Biol. 92, 753–764.

    Google Scholar 

  • Morris, P.C., Maddock, S.E., Jones, M.G.K., Bowles, D.J. (1985) Planta 166, 407–413.

    Google Scholar 

  • Murashige, T., Skoog, F. (1962) Physiol. Plant 15, 473–497.

    Google Scholar 

  • Ozias-Akins, P., Vasil, I.K. (1982) Protoplasma 110, 95–105.

    Google Scholar 

  • Ozias-Akins, P., Vasil, I.K. (1983) Amer. J. Bot. 70, 1092–1097.

    Google Scholar 

  • Raikhel, N.V., Mishkind, M.L., Palevitz, B.A. (1984) Planta 162, 55–61.

    Google Scholar 

  • Stinissen, H.M., Peumans, W.J., Carlier, A.R. (1981) Arch. Int. Physiol. Biochim. 89, B132.

  • Wernicke, W., Potrykus, I., Thomas, E. (1982) Protoplasma 111, 53–62.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Leeds, LS2 9JT, Leeds, UK

    P. C. Morris & D. J. Bowles

  2. Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK

    S. E. Maddock & M. G. K. Jones

Authors
  1. P. C. Morris
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  2. S. E. Maddock
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  3. M. G. K. Jones
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  4. D. J. Bowles
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Communicated by I. Potrykus

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Morris, P.C., Maddock, S.E., Jones, M.G.K. et al. Lectin levels in tissues of cultured immature wheat embryos. Plant Cell Reports 5, 460–463 (1986). https://doi.org/10.1007/BF00269642

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  • DOI: https://doi.org/10.1007/BF00269642

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