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Protoplast-derived streptomycin resistant plants of the forage legume Onobrychis viciifolia Scop. (sainfoin)

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Abstract

Approximately 106 protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.

Ultrastructural studies showed cells of resistant callus grown in the presence of streptomycin to contain chloroplasts with internal thykaloids and grana. Such mutant plants should be useful in designing biochemical selection schemes to recover somatic hybrids and cybrids.

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Abbreviations

BAP:

6 - benzylamino purine

NAA:

α - napthaleneacetic acid

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Author notes

  1. J. D. Hamill

    Present address: AFRC Food Research Institute, Colney Lane, NR4 7UA, Norwich, UK

  2. P. S. Ahuja

    Present address: CIMAP, RS Misra Nagar, P.O. Bag I, Lucknow, India

Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK

    J. D. Hamill, P. S. Ahuja, M. R. Davey & E. C. Cocking

Authors
  1. J. D. Hamill
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  2. P. S. Ahuja
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  3. M. R. Davey
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  4. E. C. Cocking
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Additional information

Communicated by I. Potrykus

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Hamill, J.D., Ahuja, P.S., Davey, M.R. et al. Protoplast-derived streptomycin resistant plants of the forage legume Onobrychis viciifolia Scop. (sainfoin). Plant Cell Reports 5, 439–441 (1986). https://doi.org/10.1007/BF00269636

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  • DOI: https://doi.org/10.1007/BF00269636

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