Abstract
Vacuolate mesophyll protoplasts of Nicotiana tabacum L. (cv. Samsun) were electrically fused with evacuolate protoplasts of the same species. For this purpose a mass fusion chamber was constructed. Due to distinct differences in the specific density of the respective protoplast populations, interspecific hybrids could be separated from intraspecific ones as well as from unfused parental protoplasts on an isoosmotic density gradient. The interspecific hybrids appeared to be viable to about 60% as assayed by a bacterial test for photosynthetic oxygen evolution.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bates GW, Hasenkampf CA (1985) Theor Appl Genet 70:227–233
Bornman JF, Bornman CH (1983) In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds), Protoplasts 1983, Birkhäuser Verlag, Basel, pp 208–209
Burgess J, Lawrence W (1985) Protoplasma 126:140–146
Förster E, Emeis CC (1985) FEMS Microbiology Letters 26:65–69
Fromm M, Taylor LP, Walbot V (1985) Proc Natl Acad Sci (USA) 82:5824–5828
Griesbach RJ, Sink KC (1983) Plant Sci Lett 30:297–301
Hampp R, Mehrle W, Zimmermann U (1986) Plant Physiol 81:854–858
Hampp R, Steingraber M, Mehrle W, Zimmermann U (1985) Naturwissenschaften 72:91–92
Harms CT, Potrykus I (1978a) Theor Appl Genet 53:49–55
Harms CT, Potrykus I (1978b) Theor Appl Genet 53:57–63
Koop HU, Schweiger HG (1985) Eur J Cell Biol 39:46–49
Larkin PJ (1976) Planta 128:213–216
Lazar GB (1983) In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds), Protoplasts 1983, Lecture Proc. 6th Int Protoplast Symp, Basel, 61–67
Mehrle W, Zimmermann U, Hampp R (1985) FEBS Lett 185:89–94
Nishimura M, Douce R, Akazawa T (1985) Plant Physiol 78:343–346
Verhoek-Köhler B, Hampp R, Ziegler H, Zimmermann U (1983) Planta 158:199–204
Watts J, King JM (1984) Bioscience Rep 4:335–342
Zimmermann U (1982) Biochim Biophys Acta 694:227–277
Zimmermann U, Vienken J (1982) J. Membr Biol 67:165–182
Zimmermann U, Vienken J, Halfmann J, CC Emeis (1985) Advances in Biotechnological Processes 4:79–150
Author information
Authors and Affiliations
Additional information
Communicated by M.H. Zenk
Rights and permissions
About this article
Cite this article
Naton, B., Mehrle, W., Hampp, R. et al. Mass electrofusion and mass selection of functional hybrids from vacuolate × evacuolate protoplasts. Plant Cell Reports 5, 419–422 (1986). https://doi.org/10.1007/BF00269631
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269631