Summary
By using rifampicin to increase the rate of β and β′ synthesis in a heterodiploid strain of E. coli carrying the mutation rif pr (Km7), which codes for a rifampicin sensitive RNA polymerase to which the drug binds weakly, and the dominant mutation rif RD , which codes for a rifampicin resistant RNA polymerase, the concentration of these subunits in the cell was increased 1.6 fold. Measurements made after removal of rifampicin from the cells showed that the excess β and β′ subunits did not reduce the rate of their own synthesis below normal.
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Tittawella, I.P.B. Evidence against autorepression of the ββ′ operon in Escherichia coli . Molec. Gen. Genet. 145, 223–226 (1976). https://doi.org/10.1007/BF00269598
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DOI: https://doi.org/10.1007/BF00269598