Skip to main content
SpringerLink
Log in

Rapid test for assay of ozone sensitivity in Escherichia coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

A rapid test devised for assay of ozone sensitivity in Escherichia coli is described. The detection of new mutants, either more resistant or more sensitive than wild type strain to ozone, and the genetic analysis of ozone recombinants are now possible. Results confirm that ozone resistance is probably involved with DNA repair mechanisms; and show that ozone and ultraviolet light inhibit the cell division capacity of lon mutants in a similar way.

Résumé

Une méthode permettant de déterminer rapidement la susceptibilité de Escherichia coli à l'ozone est décrite. Grâce à ce test rapide, il est maintenant possible de détecter de nouveaux mutants, plus résistants ou plus sensibles à l'ozone que la souche de type sauvage, et de procéder à l'analyse de recombinants pour ce caractère. Les résultats obtenus confirment le rôle probable des enzymes de réparation de l'ADN dans la résistance à l'ozone; et montrent que l'ozone inhibe la capacité de division cellulaire de mutants lon de façon comparable à la lumière ultraviolette.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adler, H.I., Hardigree, A.A.: Analysis of a gene controlling cell division and sensitivity to radiation in Escherichia coli. J. Bact. 87, 720–726 (1964)

    Google Scholar 

  • Broadwater, W.T., Hoehn, R.C., King, P.H.: Sensitivity of three selected bacterial species to ozone. Appl. Microbiol. 26, 391–393 (1973)

    Google Scholar 

  • Chung, Y.S., Greenberg, J.: Genes affecting sensitivity to ultraviolet light in the malB region of the chromosome of Escherichia coli. Genetics 59, 11–22 (1968)

    Google Scholar 

  • Chung, Y.S., Greenberg, J.: Complementation between exr and uvrA strains of Escherichia coli. Molec. gen. Genet. 104, 12–15 (1969)

    Google Scholar 

  • Chung, Y.S., Ofoe, E., Hamelin, C.: Localisation des gènes lex et exrA chez Escherichia coli K12. Naturaliste Can. 101, 373–375 (1975)

    Google Scholar 

  • Davis, I.: Microbiologic studies with ozone. Quantitative lethality of ozone for Escherichia coli. U.S.A.F. School of Aerospace Medicine Report 61-54, 1–16 (1961)

  • Donch, J., Green, M.H.L., Greenberg, J.: Interaction of the exr and lon genes in Escherichia coli. J. Bact. 96, 1704–1710 (1968)

    Google Scholar 

  • Donch, J., Greenberg, J.: Ultraviolet sensitivity of Escherichia coli B. J. Bact. 95, 1555–1559 (1968)

    Google Scholar 

  • Feder, W.A., Sullivan, F.: Ozone-depression of frond multiplication and floral production in duckweed. Science 165, 1373–1374 (1969)

    Google Scholar 

  • Fetner, R.H.: Mitotic inhibition induced in grasshopper neuroblasts by exposure to ozone. U.S.A.F. School of Aerospace Medicine Report 63-39 1–10 (1963)

  • Fetner, R.H., Ingols, R.S.: A comparision of the bactericidal activity of ozone and chlorine against Escherichia coli at 1°. J. gen. Microbiol. 15, 381–385 (1956)

    Google Scholar 

  • Greenberg, J., Berends, L.J., Donch, J., Green, M.H.L.: exrB: a malB linked gene in Escherichia coli B involved in sensitivity to radiation and filament formation. Genet. Res. 23, 175–184 (1974)

    Google Scholar 

  • Hamelin, C., Chung Y.S.: Résistance à l'ozone chez Escherichia coli I. Etude comparative de différentes souches B et B/r. Rev. Canad. Biol. 32, 169–176 (1973)

    Google Scholar 

  • Hamelin, C., Chung, Y.S.: Réslstance à l'ozone chez Escherichia coli II. Relations avec certains mécanismes de réparation de l'DNA. Molec. gen. Genet. 129, 177–184 (1974a)

    Google Scholar 

  • Hamelin, C., Chung, Y.S.: Optimal conditions for mutagenesis by ozone in Escherichia coli K12. Mutation Res. 24, 271–279 (1974b)

    Google Scholar 

  • Howard-Flanders, P., Simson, E., Theriot, L.: A locus that controls filament formation and sensitivity to radiation in Escherichia coli K-12. Genetics 49, 237–246 (1964)

    Google Scholar 

  • Ingram, M., Haines, R.B.: Inhibition of bacterial growth by pure ozone in the presence of nutrients. J. Hyg. (Lond.) 47, 146–158 (1949)

    Google Scholar 

  • MacLean, S., Longwell, A.C., Blogoslawski, W.J.: Effects of ozone-treated seawater on the spawned, fertilized, meiotic and cleaving eggs of the commercial american oyster. Mutation Res. 21, 283–285 (1973)

    Google Scholar 

  • Mount, D.W., Low, K.B., Edminston, S.J.: Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. J. Bact. 112, 886–893 (1972)

    Google Scholar 

  • Pace, D.M., Landolt, P.A., Alftonomos, B.T.: Effects of ozone on cells in vitro. Arch. environm. Hlth 18, 165–170 (1969)

    Google Scholar 

  • Scott, D.B.M., Lesher, E.C.: Effect of ozone on survival and permeability of Escherichia coli. J. Bact. 85, 567–576 (1963)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Département des Sciences biologiques, Université de Montréal, C.P. 6128, 101, Montréal, Canada

    Claude Hamelin & Young S. Chung

Authors
  1. Claude Hamelin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young S. Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Gros

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hamelin, C., Chung, Y.S. Rapid test for assay of ozone sensitivity in Escherichia coli . Molec. Gen. Genet. 145, 191–194 (1976). https://doi.org/10.1007/BF00269593

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269593

Keywords

Search

Navigation