Skip to main content
SpringerLink
Log in

Indirect assessment of callus fresh weight by non-destructive methods

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

The size of callus of Nicotiana plumbaginifolia was measured by determinations of fresh weight (FW), area (electronic planimeter and a point-counting method) and width (standard width and greatest width). All these methods, with the exception of the standard width measurements, were found to produce adequate substitutes for fresh weight.

Particular advantages apply to the use of the point-counting method, but the relationship between callus area and point interval was found to be critically important in determining the accuracy of measurements. The use of surface dimensions rather than FW permits continuous measurement of callus size without disturbance of the callus or its environment within the containers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

FW:

fresh weight

References

  • Barrett JP, Philbrook JS (1970) J Forestry 69: 149–151

    Google Scholar 

  • Bellhouse DR (1981) Biometrics 37: 303–312

    Google Scholar 

  • Conner AJ, Meredith CP (1984) Plant Cell Tissue Organ Culture 3: 59–68

    Google Scholar 

  • Davies DG, Stolzenberg RL, Dusky JA (1984) Weed Sci 32: 235–242

    Google Scholar 

  • Fowler CW, Janick J (1974) Hort Sci 9: 552

    Google Scholar 

  • Frolov YS, Maling DH (1969) Cart J 6: 21–35

    Google Scholar 

  • Gilissen LJW, Hannisch ten Cate CH, Keen B (1983) Plant Cell Reports 2: 232–235

    Google Scholar 

  • Grossman K, Jung J (1984) Plant Cell Reports 3: 156–158

    Google Scholar 

  • Horsch RB, King J, Jones GE (1980) Can J Bot 58: 2402–2406

    Google Scholar 

  • Keen B (1986) Technical and Preliminary Research Report, Research Institute ITAL, Wageningen

  • Lloyd PR (1976) Cart J 13: 22–26

    Google Scholar 

  • Márton L, Dung TM, Mendel RR, Maliga P (1982) Mol Gen Get 182: 301–304

    Google Scholar 

  • Nabors MW, Kroskey CS, McHugh DM (1982) Z Pflanzenphysiol 105: 341–349

    Google Scholar 

  • Sloan ME, Camper ND (1981) Pest. Biochem. Physiol. 15: 201–208

    Google Scholar 

  • Street HE (1977) In: Street HE (Ed) Botanical Monographs Vol 11, Blackwell Scientific Publications, Oxford, pp 61–102

    Google Scholar 

  • Toth R (1982) Amer. J. Bot. 69: 1694–1706.

    Google Scholar 

Download references

Author information

Author notes

  1. J. Mottley

    Present address: Department of Biology and Biochemistry, North East London Polytechnic, Romford Road, E15 4LZ, London, UK

  2. B. Keen

    Present address: Institute voor Toegepaste Informatica TNO, Postbus 100, NL-6700 AC, Wageningen, The Netherlands

Authors and Affiliations

  1. ITAL Research Institute, P.O. Box 48, NL-6700, Wageningen, The Netherlands

    J. Mottley & B. Keen

Authors
  1. J. Mottley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Keen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Barz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mottley, J., Keen, B. Indirect assessment of callus fresh weight by non-destructive methods. Plant Cell Reports 6, 389–392 (1987). https://doi.org/10.1007/BF00269569

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269569

Keywords

Search

Navigation