Abstract
Karyotypic studies on cell suspensions and calli of an S-(2-aminoethyl)cysteine resistant cell line of the interdihaploid potato H2578 (2n=24) revealed a high degree of variation in the number of chromosomes (33–217) and dicentric chromosomes (0–8). The suspensions also exhibited megachromosomes and fused chromosomes. Differential staining showed that in suspensions dicentrics survived mitotic cycles mainly by parallel separation of the chromatids during anaphase and hardly by the breakage-fusion-bridge cycle. Two phenotypes of plantlets regenerated, each with 34 or 35 chromosomes with gross structural mutations and with the triploid amount of DNA. Chromosomal variation was related to the degree of tissue organization.
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Bokelmann GS, Roest S (1983) Z. Pflanzenphysiol. 109: 259–265
Brewen JG, Peacock WJ (1969) Proc. Natl. Acad. Sci. USA 62: 389–394
Browers MA, Orton TJ (1982) J. Hered. 73: 159–162
Burns JA, Gerstel DU (1973) Genetics 75: 497–502
Carrano AV, Heddle JA (1973) J. Theor. Biol. 38: 289–304
D'Amato F (1985) CRC Crit. Rev. Plant Sci. 3: 73–112
Doležel J, Novák FJ (1986) Plant Cell Reports 5: 280–283
Evans DA, Gamborg OL (1982) Plant Cell Reports 1: 104–107
Fish N, Karp A (1986) Theor. Appl. Genet. 72: 405–412
Gill BS, Kam-Morgan LNW, Shepard JF (1987) J. Hered. 78: 15–20
Harms CT, Potrykus I, Widholm JM (1981) Z. Pflanzenphysiol. 101: 377–390
Jacobsen E (1986) J. Plant Physiol. 123: 307–315
Kao KN, Miller RA, Gamborg OL, Harvey BL (1970) Can. J. Genet. Cytol. 12: 297–301
Karp A, Bright WJ (1985) Oxford Surveys Plant Mol. Cell Biol. 2: 199–234
Kovács EI (1985) Theor. Appl. Genet. 70: 548–554
Murata M, Orton TJ (1984) Can. J. Genet. Cytol. 26: 395–400
Mouras A (1984) Differentiation 27: 88–93
Pijnacker LP, Ferwerda MA (1984) Can. J. Genet. Cytol. 26: 415–419
Pijnacker LP, Hermelink JHM, Ferwerda MA (1986)a Plant Cell Reports 5: 43–46
Pijnacker LP, Walch K, Ferwerda MA (1986)b Theor. Appl. Genet. 72: 833–839
Sree Ramulu K (1986) In: Vasil IK (ed) Cell culture and somatic cell genetics of plants 3: 449–473
Sree Ramulu K, Dijkhuis P, Hänisch ten Cate ChH, Groot B de (1985) Plant Science 41: 69–78
Sumner AT (1972) Exp. Cell Res. 75: 304–306
Sunderland N (1977) In: Street HE (ed) Plant tissue and cell culture, Blackwell Sci. Publ., Oxford, pp. 177–205
Tempelaar MJ (1980) Chromosoma 77: 359–371
Toncelli F, Martini G, Giovinazzo G, Nuti Ronchi V (1985) Theor. Appl. Genet. 70: 345–348
Vries SE de, Bokelmann GS (1986) J. Plant Physiol. 122: 199–203
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Communicated by I. Potrykus
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Pijnacker, L.P., Ferwerda, M.A. Karyotype variation in aminoethylcysteine resistant cell and callus cultures and regenerated plants of a dihaploid potato (Solanum tuberosum). Plant Cell Reports 6, 385–388 (1987). https://doi.org/10.1007/BF00269568
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DOI: https://doi.org/10.1007/BF00269568