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Plant regeneration from protoplast culture of sweet potato (Ipomoea batatas Lam.)

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Abstract

This is the first report on successful plant regeneration from protoplasts of sweet potato. Two cultivars (Guyana and Duclos XI) of sweet potato plants propagated under in vitro conditions were used as the source of protoplasts. Green compact calli with meristematic areas were induced in the medium supplemented with 2mg1−1 zeatin, and plant regeneration occurred when these calli were transferred onto the medium with zeatin level reduced to 0.25mg1−1. Plant regeneration was found to be genotype-dependent, since it was only obtained for cultivar Duclos XI.

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Abbreviations

MS:

Murashige and Skoog basal medium

IAA:

Indol-3-acetic acid

NAA:

naphthaleneacetic acid

2,4-D:

dichlorophenoxyacetic acid

Mes:

2-(N-morpholino)-ethanesulfonic acid

Cpw:

cell and protoplast washing solution

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Authors and Affiliations

  1. Laboratoire de Morphogénèse Végétale Expérimentale, associé au CNRS (UA115), Bât 360, Université de Paris XI, Centre d'Orsay, F-91405, Orsay, France

    Darasinh Sihachakr & Georges Ducreux

Authors
  1. Darasinh Sihachakr
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  2. Georges Ducreux
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Communicated by A. M. Boudet

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Sihachakr, D., Ducreux, G. Plant regeneration from protoplast culture of sweet potato (Ipomoea batatas Lam.). Plant Cell Reports 6, 326–328 (1987). https://doi.org/10.1007/BF00269551

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  • DOI: https://doi.org/10.1007/BF00269551

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