Abstract
We have adapted the chemical DNA sequencing method developed by Maxam and Gilbert to study human metaphase chromosomes. Particularly, the in situ cytosine-specific chemical cleavage has been assayed. After exhaustive treatments, a C-like banding pattern was consistently shown. Since methyl groups interfere with the cytosine-specific chemical cleavage reaction, we assume that heterochromatin remains resistant as a result of the high level of methylation. On the other hand when moderate treatments are applied, T-bands remain as resistant regions, implying that these domains would be the most intensely methylated regions within euchromatin.
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De Cabo, S.F., Fernández-Piqueras, J. & Visedo, G. An adaptation of the maxam and gilbert sequencing method to the study of human metaphase chromosomes. Histochemistry 101, 205–207 (1994). https://doi.org/10.1007/BF00269545
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DOI: https://doi.org/10.1007/BF00269545