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In vitro plant regeneration of leguminous trees (Albizia spp)

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Abstract

Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardiana (1.6). Higher concentrations of auxins in the medium, in general, enhanced rooting and callusing but cytokinins promoted the growth of green calli. BA enchanced the differentiation of shoots in the three species. The in vitro grown shoots of A. amara and A. richardiana, after subculturing on B5+1 μM IAA developed roots (up to 30–40%). These plants have been successfully transferred to the field.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

6-benzylaminopurine

BM:

Gamborg's B5 medium with 0.9% agar+3% sucrose

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kn:

Kinetin

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. Department of Botany, University of Delhi, 110007, Delhi, India

    Uttar K. Tomar & Shrish C. Gupta

Authors
  1. Uttar K. Tomar
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  2. Shrish C. Gupta
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Communicated by J. M. Widholm

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Tomar, U.K., Gupta, S.C. In vitro plant regeneration of leguminous trees (Albizia spp). Plant Cell Reports 7, 385–388 (1988). https://doi.org/10.1007/BF00269518

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  • DOI: https://doi.org/10.1007/BF00269518

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