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The organization of genes in yeast mitochondrial DNA

II. The physical map of EcoRI and HindII+III fragments

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Summary

  1. 1.

    We have isolated large fragments of the mtDNA of the yeast Saccharomyces carlsbergensis and digested these with restriction endonucleases. The digestion products were separated by electrophoresis in agarose gels.

  2. 2.

    Endonucleases EcoRI, HindII+III, HpaI, HindIII and HapII yield 9, 11, 6, 0 and >80 fragments, respectively.

  3. 3.

    By analysis of partial digestion products and by redigesting the fragments obtained with one endonuclease with a second, we have established the order of all EcoRI and HindII+III fragments. The map is circular and its contour length is 22.1±0.35 μm, in good agreement with earlier estimates of the size of yeast mtDNA, using electron microscopy and renaturation kinetics.

  4. 4.

    A comparison of the fragmentation pattern of mtDNAs from S. carlsbergensis and various strains of Saccharomyces cerevisiae with endonuclease HindII+III suggests that the overall gene order is similar.

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  1. Section for Medical Enzymology and Molecular Biology, Laboratory of Biochemistry, University of Amsterdam, Eerste Constantijn Huygensstraat 20, Amsterdam, (The Netherlands)

    Johan P. M. Sanders, Piet Borst & Peter J. Weijers

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  1. Johan P. M. Sanders
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  2. Piet Borst
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Communicated by F. Kaudewitz

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Sanders, J.P.M., Borst, P. & Weijers, P.J. The organization of genes in yeast mitochondrial DNA. Molec. Gen. Genet. 143, 53–64 (1975). https://doi.org/10.1007/BF00269420

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