Summary
The fate of mitochondrial DNA, following mating between a grande and suppressive petite of Saccharomyces cerevisiae, has been followed for up to 60 generations. The buoyant density of the mitochondrial DNA was seen to change in a manner explicable by a combination of recombination and subsequent modification phenomena whilst the suppressivity of the petite zygotic clones always remained high.
These findings are consistent with current models of mitochondrial DNA metabolism in which petite strains have been observed to undergo deletion and reamplification of certain parts of their genomes.
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Blamire, J., Cryer, D.R., Finkelstein, D.B., Marmur, J.: Sedimentation properties of yeast nuclear and mitochondrial DNA. J. Mol. Biol. 67, 11–24 (1972)
Borst, P.: Mitochondrial nucleic acids. Ann. Rev. Biochem. 41, 333–337 (1972)
Clark-Walker, G.D., Miklos, G.L.G.: Complementation in cytoplasmic petite mutants of yeast to form respiratory competent cells. Proc. Natl. Acad. Sci. 72, 372–375 (1975)
Coen, D., Deutsch, J., Netter, P., Petrochillo, E., Slonimski, P.P.: Mitochondrial Genetics I. Methodology and phenomenology. In: P.L. Miller, Control of Organelle Development: 24th Symp. Soc. Exp. Biol., London, p. 449–490. Cambridge: University Press 1970
Faye, G., Fukuhara, H., Grandchamp, C., Lazowska, J., Michel, F., Casey, J., Getz, G.S., Locker, J., Rabinowitz, M., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Dujon, G., Nitter, P., Slonimski, P.P.: Mitochondrial mutants: deletions and repetitions of genes. Biochimie 55, 779–792 (1973)
Gordon, P., Rabinowitz, M.: Evidence for deletion and changed sequence in the mitochondrial DNA of a spontaneously generated petite mutant of S. cerevisiae. Biochemistry 12, 116–183 (1973)
Hollenberg, C.P., Borst, P., Van Brugger, E.F.J.: Mitochondrial DNA V. A 25 u closed circular duplex DNA molecule in wild type yeast mitochondria. Structure and genetic complexity. Biochim. Biophys. Acta (Aust) 209, 1–15 (1970)
Horack, I., Coon, D.G., David, I.B.: Interspecific recombination of mitochondrial DNA molecules in hybrids of somatic cells. Proc. Natl. Acad. Sci. 71, 1828–1833 (1974)
Jakob, H.: Technique de synchronisation de la formation des zygotes chez la levure Saccharomyces cerevisiae. Compt. Rend. Acad. Sci. Paris 254, 3909–3911 (1962)
Locker, J., Rabinowitz, M., Getz, G.S.: Tandem inverted repeats in mitochondrial DNA of petite mutants of S. cerevisiae. Proc. Nat. Acad. Sci. U.S.A. 71, 1366–1370 (1974)
Michaelis, G., Petrochillo, E., Slonimski, P.P.: Mitochondrial Genetics III. Recombined molecules of mitochondrial DNA obtained from crosses between cytoplasmic petite mutants of S. cerevisiae: physical and genetic characterization. Mol. Gen. Genet. 123, 51–65 (1973)
Michels, C.A., Blamire, J., Goldfinger, B., Marmur, J.: Studies on the action of nalidixic acid in the yeast S. cerevisiae. Antimicrobial Agents and Chemotherapy 3, 562–567 (1973)
Michels, C.A., Blamire, J., Goldfinger, B., Marmur, J.: A genetic and biochemical analysis of petite mutations in yeast. J. Mol. Biol. 90, 431–449 (1974)
Mol, J.N.M., Borst, P., Grosveld, F.G., Spencer, J.H.: The size of the repeating unit of the repetitive mitochondrial DNA from a “low density” petite mutant of yeast. Biochim. Biophys. Acta 374, 115–118 (1974)
Preer, J.R., Jr.: Extrachromosomal inheritance: hereditary symbionts, mitochondria, chloroplasts. Ann. Rev. Genetics 5, 361–406 (1971)
Rank, G.H.: Genetic evidence for ‘Darwinian’ selection at the molecular level. II. Genetic analysis of cytoplasmically-inherited high and low suppressivity in Saccharomyces cerevisiae. Can. J. Genet. Cytol, 12, 340–346 (1970)
Saunders, J.P., Flavell, R.A., Borst, P., Mol, J.N.M.: Nature of the base sequence conserved in the mitochondrial DNA of a low density petite. Biochim. Biophys. Acta 312, 441–457 (1973)
Shannon, C.A., Rao, A., Douglass, S., Criddle, R.S.: Recombination in yeast mitochondrial DNA. J. Supramol. Structure 1, 145–152 (1972)
Shatz, G., Mason, T.L.: The biosynthesis of mitochondrial protein. Ann. Rev. Biochem. 43, 51–88 (1974)
Thomas, D.Y., Wilkie, D.: Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controling resistance. Genet. Res. 11, 33–41 (1968)a
Thomas, D.Y., Wilkie, D.: Recombination of mitochondrial drug resistance factors in S. cerevisiae. Biochem. Biophys. Res. Comm. 30, 368–372 (1968)b
Wilkie, D., Saunders, G., Linnane, A.W.: Inhibition of respiratory enzyme synthesis in yeast by chloramphenicol: Relationship between chloramphenicol tolerance and resistance to other antibacterial antibiotics. Genet. Res. Camb. 10, 199–203 (1967)
Williamson, D.H., Fennell, D.J.: Apparent dispersive replication of yeast mitochondrial DNA as revealed by density labelling experiments. Molec. Gen. Genetics 131, 193–207 (1974)
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Communicated by Ch. Auerbach
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Blamire, J., Michels, C.A., Walsh, J.M. et al. Mitochondrial DNA in yeast recombination and subsequent modification following mating between a grande and a suppressive petite. Molec. Gen. Genet. 143, 253–259 (1976). https://doi.org/10.1007/BF00269401
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DOI: https://doi.org/10.1007/BF00269401