Summary
A strain carrying the ilv0603 mutation has been isolated in E. coli K-12 and its characteristics were found to be very similar to those previously reported by Ramakrishnan and Adelberg (1965a) for other ilv0 mutants.
The strain carrying the ilv0603 mutation is resistant to valine inhibition (Valr) and we show that this resistance depends on the expression of a newly recognized gene, ilvG, which is located at min 75, between ilvE and ilvD on the E. coli K-12 map. The ilvG gene causes the expression of a Valr acetolactate synthase, which is detectable only when the ilv0603 mutation is also present in cis on the same chromosome. Under these conditions the Valr acetolactate synthase activity is eluted, on a hydroxylapatite column, at an ionic strength slightly lower than that required for elution of the remaining acetolactate synthase activity (sensitive to valine inhibition). The Valr peak is missing in a strain carrying an ilvG (amber) mutation.
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References
Andoh, T., Ozeki, H.: Suppressor gene su3+ of E. coli, a structural gene for tyrosine tRNA. Proc. nat. Acad. Sci. (Wash.) 59, 792–799 (1968)
Avitabile, A., Carlomagno-Cerillo, M.S., Favre, R., Blasi, F.: Isolation of transducing bacteriphages for the histidine and isoleucine-valine operons in Escherichia coli K-12. J. Bact. 112, 40–47 (1972)
Blatt, J.M., Pledger, W.J., Umbarger, H.E.: Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase. Biochem. biophys. Res. Commun. 48, 444–450 (1972)
Calhoun, D.H., Kuska, J., Hatfield, G.W.: Threonine deaminase from Escherichia coli. II. Maturation and physical properties of the enzyme from a mutant altered in its regulation of gene expression. J. biol. Chem. (in press)
Cervone, F., Iaccarino, M.: The action of 0-methyl-threonine and thiaisoleucine on threonine deaminase purified from Escherichia coli K-12. FEBS Letters 26, 56–60 (1972)
De Felice, M., Guardiola, J., Esposito, B., Iaccarino, M.: Structural genes for a newly recognized acetolactate synthase in Escherichia coli K-12. J. Bact. 120, 1068–1077 (1974)
De Felice, M., Guardiola, J., Lamberti, A., Iaccarino, M.: Escherichia coli K-12 mutants altered in he transport systems for oligo- and dipeptides. J. Bact. 116, 751–756 (1973)
De Felice, M., Guardiola, J., Malorni, M.C., Klopotowski, T., Iaccarino, M.: Regulation of the pool size of valine in Escherichia coli K-12. J. Bact. 120, 1058–1067 (1974)
Dwyer, S.B., Umbarger, H.E.: Isoleucine and valine metabolism of Escherichia coli. XVI. Pattern of multivalent repression in strain K-12. J. Bact. 95, 1680–1684 (1968)
Favre, R., Iaccarino, M., Levinthal, M.: Complementation between different mutations in the ilvA gene of Escherichia coli K-12. J. Bact. 119, 1069–1071 (1974)
Glover, S.W.: Valine resistant mutants of Escherichia coli K-12. Genet. Res. 3, 448–460 (1962)
Gorini, L., Kaufman, H.: Selecting bacterial mutants by the penicillin method. Science 131, 604–605 (1960)
Guardiola, J., Iaccarino, M.: Escherichia coli K-12 mutants altered in the transport of branched-chain amino acids. J. Bact. 108, 1034–1044 (1971)
Guardiola, J., Felice, M. de, Klopotowski, T., Iaccarino, M.: Multiplicity of isoleucine, leucine and valine transport systems in Escherichia coli K-12. J. Bact. 117, 382–392 (1974a)
Guardiola, J., Felice, M. de, Klopotowski, T., Iaccarino, M.: Mutations affecting the different transport systems for isoleucine, leucine and valine in Escherichia coli K-12. J. Bact. 117, 393–405 (1974b)
Guardiola, J., Felice, M. de, Iaccarino, M.: Mutant of Escherichia coli K-12 missing acetolactate synthase activity. J. Bact. 120, 536–538 (1974)
Iaccarino, M., Berg., P.: Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase. J. Bact. 105, 527–537 (1971)
Kiritani, K., Matsuno, T., Ikeda, Y.: Genetic and biochemical studies on isoleucine and valine requiring mutants of Escherichia coli. Genetics 51, 341–349 (1965)
Kline, E.L., Brown, C.S., Coleman, W.G. Jr., Umbarger, H.E.: Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12. Biochem. biophys. Res. Commun. 57, 1144–1151 (1974)
Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206 (1955)
Lo Schiavo, F., Favre, R., Kasai, T., Cascino, A., Guardiola, J., Caro, L., Iaccarino, M.: The ϕ80λdilv phage and its use to detect mRNA related to isoleucine-valine metabolism. J. molec. Biol. (in press)
Marsh, N.J., Duggan, D.E.: Ordering of mutant sites in the isoleucine-valine genes of Escherichia coli by use of merogenotes derived from F14: a new procedure for fine structure mapping. J. Bact. 109, 730–740 (1972)
O'Neill, J.P., Freundlich, M.: Two forms of biosynthetic acetohydroxy acid synthetase in Salmonella typhimurium. Biochem. biophys. Res. Commun., 48, 437–443 (1972)
O'Neill, J.P., Freundlich, M.: Temperature-sensitive growth inhibition by valine in Salmonella typhimurium: alteration of one form of acetohydroxy acid synthetase. J. Bact. 116, 98–106 (1973)
Ramakrishnan, T., Adelberg, E.A.: Regulatory mechanisms in the biosynthesis of isoleucine and valine. I. Genetic derepression of enzyme formation. J. Bact. 87, 566–573 (1964)
Ramakrishnan, T., Adelberg, E.A.: Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J. Bact. 89, 654–660 (1965a)
Ramakrishnan, T., Adelberg, E.A.: Regulatory mechanisms in the biosynthesis of isoleucine and valine. III. Map order of the structural genes and operator genes. J. Bact. 89, 661–664 (1965b)
Taylor, A.L., Trotter, C.D.: Linkage map of Escherichia coli strain K-12. Bact. Rev. 36, 504–524 (1972)
Umbarger, H.E.: In: Current topics in Cellular Regulation (B.L. Horecker and E.R. Stadtman, eds.), vol. 1, p. 57. London-New York: Academic Press 1969
Vogel, H.J., Bonner, D.M.: Acetylornithinase of Escherichia coli: partial purification and some properties. J. biol. Chem. 218, 97–106 (1956)
Wasmuth, J.J., Umbarger, H.E.: Role for free isoleucine or glycylleucine in the repression of threonine deaminase in Escherichia coli. J. Bact. 117, 29–39 (1974)
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Favre, R., Wiater, A., Puppo, S. et al. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12. Molec. Gen. Genet. 143, 243–252 (1976). https://doi.org/10.1007/BF00269400
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DOI: https://doi.org/10.1007/BF00269400