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Sorghum plant regeneration from aluminum selection media

Summary

Callus cultures of four Sorghum bicolor (L.) Moench cultivars were initiated from aseptically germinated seed. Prior to culture on media containing 0, 100, 200, and 400 μM aluminum, the callus had been subcultured for 3 to 12 months. There were differences amongst the cultivars in response to the aluminum in terms of callus growth. Growth was inhibited at the highest level of aluminum tested. Subculture of callus from aluminum selection medium on both aluminum-containing and aluminum-free media indicated that aluminum-selected callus grew better in the presence of aluminum. Plants were regenerated from only one cultivar (IS3620C of the Margaritiferum group) after several passages on aluminum-supplemented media. Regeneration capacity was however inhibited at 400 μM aluminum.

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References

  • Brettell RIS, Wernicke W, Thomas E (1980) Protoplasma 104:141–148

    Google Scholar 

  • Croughan TP, Stavarek SJ, Rains DW (1978) Crop Sci 18:959–963

    Google Scholar 

  • — (1981) Env and Expt Bot 21:317–324

    Google Scholar 

  • Dix PJ, Street HE (1975) Plant Sci Lett 5:231–237

    Google Scholar 

  • Foy CD, Chaney RL, White MC (1978) Ann Rev Plant Physiol 29:511–566

    Google Scholar 

  • Furlani PR, Clark RB (1981) Agro J 73:587–594

    Google Scholar 

  • Hasegawa PM, Bressan RA, Handa AK (1980) Plant and Cell Physiol 21:1347–1355

    Google Scholar 

  • Heller R (1949) Compt Rend Soc Biol 143:335

    Google Scholar 

  • Mathur AK, Ganapathy PS, Johri BM (1980) Z Pflanzenphysiol 99:287–294

    Google Scholar 

  • Meredith CP (1978) Plant Sci Lett 12:17–24

    Google Scholar 

  • — (1978) Plant Sci Lett 12:25–34

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    Google Scholar 

  • Murty B, Arunachalam RV, Saxena MB (1967) Indian J Genet Plant Breed 27:1–74

    Google Scholar 

  • Nabors MW, Daniels A, Nadolny L, Brown C (1975) Plant Sci Lett 4:155–159

    Google Scholar 

  • —, Gibbs SE, Bernstein CS, Meis ME (1980) Z Pflanzenphysiol 97:13–17

    Google Scholar 

Download references

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Technical Article 18057 from the Texas Agricultural Experiment Station TAM 22 AID/DSAN/XII/G-0149 from USAID

Communicated by O. Gamborg

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Smith, R.H., Bhaskaran, S. & Schertz, K. Sorghum plant regeneration from aluminum selection media. Plant Cell Reports 2, 129–132 (1983). https://doi.org/10.1007/BF00269336

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  • DOI: https://doi.org/10.1007/BF00269336

Keywords

  • Aluminum
  • Germinate
  • Selection Medium
  • Plant Regeneration
  • Callus Culture