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Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine

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Abstract

Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml aphidicolin for 18 h, then cultured in drug-free medium for 8 h and finally exposed to 0.1% colchicine for 8 additional hours exhibited MI of 62.8% and 65.7% respectively, for the two cell lines in study.

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Abbreviations

MI:

mitotic index

2,4-D:

2,4-dichlorophenoxyacetic acid

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Authors and Affiliations

  1. Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, 50 250, Bet Dagan, Israel

    Assaf Guri, Aaron Zelcer & Shamay Izhar

Authors
  1. Assaf Guri
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  2. Aaron Zelcer
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  3. Shamay Izhar
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Additional information

Communicated by F. Constabel

Contribution from the Agricultural Research Organization, The Volcani Center, Bet-Dagan 50-250, Israel No. 1028-E, 1984 series.

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Guri, A., Zelcer, A. & Izhar, S. Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine. Plant Cell Reports 3, 219–221 (1984). https://doi.org/10.1007/BF00269296

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  • DOI: https://doi.org/10.1007/BF00269296

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