Abstract
Photobiont cells of the lichen Evernia prunastri have completely been separated from their fungal partner by filtration through a bed of Sepharose 2B. Both mannitol and ribitol have been quantified by gas-liquid chromatography in the different steps of the isolation procedure. Absence of mannitol, which is exclusively produced by the mycobiont, has been used as the best probe to monitor isolation.
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Communicated by D. von Wettstein
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Pérez, M.J., Vicente, C. & Legaz, M.E. An improved method to isolate lichen algae by gel filtration. Plant Cell Reports 4, 210–211 (1985). https://doi.org/10.1007/BF00269291
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DOI: https://doi.org/10.1007/BF00269291