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Involvement of some host function of Escherichia coli in the turn off control of phages ϕ80 and λ

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Summary

When E. coli cells are infected with ϕ80 or its derivatives, synthesis of the viral early l mRNA is subjected to turn off “control”. We confirmed this phenomena by using several strains of E. coli K12 as the host bacteria. However, strain A19 and its derivatives were exceptions in that they did not permit the turn off control of synthesis of the ϕ80 early l mRNA. The host function showing this Tob− (negative “turn off in bacteria”) phenomenon has no relation with the rns and rel genes carried by the A19 strains. The Tob− phenotype of these strains was also observed when phage λ was infected. This suggests that action of some host factor involved in the turn off control is common for both ϕ80 and λ. Preliminary mapping was attemted to determine the bacterial gene directing the host factor.

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Authors and Affiliations

  1. The Institute for Virus Research, Kyoto University, Kyoto, Japan

    Hidetoshi Inoko & Mutsuo Imai

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  1. Hidetoshi Inoko
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  2. Mutsuo Imai
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Communicated by H. Ozeki

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Inoko, H., Imai, M. Involvement of some host function of Escherichia coli in the turn off control of phages ϕ80 and λ. Molec. Gen. Genet. 129, 49–59 (1974). https://doi.org/10.1007/BF00269265

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  • DOI: https://doi.org/10.1007/BF00269265

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